%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Kazuo Okemoto, Keiko Maekawa, Yoko Tajima, Masahiro Tohkin, Yoshiro Saito
Cross-Classification of Human Urinary Lipidome by Sex, Age, and Body Mass Index
www.plosone.org
www.plosone.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 11 0 R/Annots 12 0 R/Contents 13 0 R/TrimBox[0 0 612 792]>>
endobj
12 0 obj
[14 0 R 15 0 R 16 0 R 17 0 R 18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R]
endobj
14 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref002)>>
endobj
15 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref004)>>
endobj
16 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref005)>>
endobj
17 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref007)>>
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref008)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref009)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref011)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref012)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref013)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref014)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref015)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.t001)>>
endobj
26 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s002)>>
endobj
13 0 obj
[27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R 33 0 R 34 0 R 35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R]
endobj
27 0 obj
<>stream
q
0.83 0.64 0.02 0 k
220.989 667.3323 m
225.7512 667.3323 l
h
f*
230.9102 667.3323 m
235.6724 667.3323 l
h
f*
377.8583 628.3276 m
382.6205 628.3276 l
h
f*
387.7795 628.3276 m
392.5417 628.3276 l
h
f*
512.4472 602.3055 m
517.2094 602.3055 l
h
f*
338.9669 537.3354 m
343.7291 537.3354 l
h
f*
348.8882 537.3354 m
358.4126 537.3354 l
h
f*
489.9969 498.3307 m
499.5213 498.3307 l
h
f*
231.8173 420.3213 m
241.3417 420.3213 l
h
f*
245.8205 420.3213 m
255.3449 420.3213 l
h
f*
293.6126 172.9134 m
303.137 172.9134 l
h
f*
540.1134 94.9039 m
569.3102 94.9039 l
h
f*
217.3039 81.9213 m
251.2063 81.9213 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 211.9748 707.4141 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Owing)Tj
2.9366 0 Td
(to)Tj
1.0205 0 Td
(their)Tj
2.109 0 Td
(considerable)Tj
5.261 0 Td
(structural)Tj
4.0819 0 Td
(diversity,)Tj
3.8664 0 Td
(lipids)Tj
2.4095 0 Td
(are)Tj
1.4513 0 Td
(important)Tj
4.2803 0 Td
(players)Tj
3.0274 0 Td
(in)Tj
1.0374 0 Td
(a)Tj
0.652 0 Td
(variety)Tj
2.9253 0 Td
(of)Tj
-36.2548 -1.3039 Td
(complex)Tj
3.6226 0 Td
(physiological)Tj
5.4425 0 Td
(processes,)Tj
4.1952 0 Td
(where)Tj
2.6476 0 Td
(they)Tj
1.9275 0 Td
(execute)Tj
3.2031 0 Td
(important)Tj
4.2803 0 Td
(functions,)Tj
4.218 0 Td
(acting)Tj
2.6532 0 Td
(as)Tj
1.0148 0 Td
(cellular)Tj
-33.2048 -1.2982 Td
(membrane)Tj
4.5637 0 Td
(components,)Tj
5.4028 0 Td
(signaling)Tj
3.8211 0 Td
(mediators,)Tj
4.456 0 Td
(energy)Tj
2.9083 0 Td
(reserve)Tj
3.0444 0 Td
(molecules,)Tj
4.4277 0 Td
(and)Tj
1.7291 0 Td
(endocrine)Tj
4.2633 0 Td
(regula-)Tj
-34.6164 -1.2983 Td
(tors)Tj
1.7574 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(4)Tj
0 g
(].)Tj
2.5909 0 Td
(The)Tj
1.7858 0 Td
(so-called)Tj
3.736 0 Td
(lipid)Tj
2.0466 0 Td
(mediators)Tj
4.2293 0 Td
(are)Tj
1.4513 0 Td
(a)Tj
0.652 0 Td
(group)Tj
2.6078 0 Td
(of)Tj
1.0148 0 Td
(regulatory)Tj
4.32 0 Td
(molecules)Tj
4.2009 0 Td
(produced)Tj
4.0422 0 Td
(in)Tj
-34.435 -1.2983 Td
(response)Tj
3.7303 0 Td
(to)Tj
1.0261 0 Td
(extracellular)Tj
5.1307 0 Td
(stimuli)Tj
3.0104 0 Td
(that)Tj
1.7745 0 Td
(bind)Tj
2.0692 0 Td
(to)Tj
1.0262 0 Td
(their)Tj
2.1089 0 Td
(cognate)Tj
3.3222 0 Td
(receptors)Tj
3.9175 0 Td
(and)Tj
1.7291 0 Td
(transmit)Tj
3.617 0 Td
(signals)Tj
2.9027 0 Td
(to)Tj
-35.3648 -1.3039 Td
(target)Tj
2.5114 0 Td
(cells;)Tj
2.143 0 Td
(their)Tj
2.109 0 Td
(deregulation)Tj
5.261 0 Td
(has)Tj
1.5421 0 Td
(been)Tj
2.1203 0 Td
(linked)Tj
2.7325 0 Td
(to)Tj
1.0262 0 Td
(various)Tj
3.1408 0 Td
(pathological)Tj
5.0909 0 Td
(conditions)Tj
4.4788 0 Td
(such)Tj
2.0579 0 Td
(as)Tj
-34.2139 -1.2982 Td
(inflammation,)Tj
5.9243 0 Td
(atherosclerosis,)Tj
6.3269 0 Td
(and)Tj
1.7291 0 Td
(diabetes)Tj
3.4639 0 Td
([)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(].)Tj
2.5909 0 Td
(Lipid)Tj
2.3414 0 Td
(mediators)Tj
4.2293 0 Td
(include)Tj
3.1804 0 Td
(polyunsaturated)Tj
-29.7862 -1.2983 Td
(fatty)Tj
1.9898 0 Td
(acids)Tj
2.2337 0 Td
(\(PUFAs\),)Tj
3.9969 0 Td
(lysophosphatidic)Tj
6.9618 0 Td
(acid,)Tj
2.0976 0 Td
(and)Tj
1.7292 0 Td
(their)Tj
2.1089 0 Td
(derivatives.)Tj
4.7282 0 Td
(It)Tj
0.8617 0 Td
(is)Tj
0.8447 0 Td
(believed)Tj
3.4923 0 Td
(that)Tj
1.7801 0 Td
(many)Tj
2.4775 0 Td
(regu-)Tj
-35.3024 -1.3039 Td
(latory)Tj
2.5284 0 Td
(activities)Tj
3.7191 0 Td
(of)Tj
1.0148 0 Td
(PUFAs)Tj
3.084 0 Td
(are)Tj
1.4514 0 Td
(mediated)Tj
3.9401 0 Td
(by)Tj
1.1792 0 Td
(their)Tj
2.109 0 Td
(oxidation)Tj
4.0421 0 Td
(products,)Tj
3.9799 0 Td
(oxylipins)Tj
3.855 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(],)Tj
1.5988 0 Td
(which)Tj
2.6532 0 Td
(are)Tj
-35.155 -1.2983 Td
(produced)Tj
4.0421 0 Td
(and)Tj
1.7348 0 Td
(activated)Tj
3.7814 0 Td
(by)Tj
1.1792 0 Td
(several)Tj
2.9367 0 Td
(enzymes)Tj
3.6623 0 Td
(such)Tj
2.058 0 Td
(as)Tj
1.0148 0 Td
(cytochrome)Tj
4.9776 0 Td
(P450)Tj
2.2053 0 Td
(\(P450\),)Tj
3.1124 0 Td
(cyclooxygenases)Tj
-30.7046 -1.2983 Td
(\(COX\),)Tj
3.1861 0 Td
(and)Tj
1.7291 0 Td
(lipoxygenases)Tj
5.6976 0 Td
(\(LOX\).)Tj
3.0614 0 Td
(Oxylipins)Tj
4.0932 0 Td
(have)Tj
2.0636 0 Td
(been)Tj
2.1203 0 Td
(mostly)Tj
2.9083 0 Td
(associated)Tj
4.2576 0 Td
(with)Tj
1.9956 0 Td
(the)Tj
1.4683 0 Td
(initiation)Tj
-32.5811 -1.3039 Td
(and)Tj
1.7291 0 Td
(resolution)Tj
4.2802 0 Td
(of)Tj
1.0148 0 Td
(inflammation)Tj
5.6976 0 Td
(and)Tj
1.7292 0 Td
(vascular)Tj
3.4696 0 Td
(function.)Tj
3.855 0 Td
(The)Tj
1.7859 0 Td
(kidneys)Tj
3.2938 0 Td
(have)Tj
2.0636 0 Td
(the)Tj
1.4683 0 Td
(highest)Tj
3.0898 0 Td
(levels)Tj
2.3754 0 Td
(of)Tj
-35.8523 -1.2982 Td
(P450,)Tj
2.432 0 Td
(COX,)Tj
2.5059 0 Td
(and)Tj
1.7291 0 Td
(LOX)Tj
2.16 0 Td
(enzymes)Tj
3.6623 0 Td
(after)Tj
2.0353 0 Td
(the)Tj
1.4683 0 Td
(liver;)Tj
2.211 0 Td
(these)Tj
2.2507 0 Td
(enzymatic)Tj
4.303 0 Td
(pathways)Tj
3.9344 0 Td
(play)Tj
1.8709 0 Td
(a)Tj
0.652 0 Td
(pivotal)Tj
2.948 0 Td
(role)Tj
1.7688 0 Td
(in)Tj
-35.9317 -1.2983 Td
(kidney)Tj
2.9366 0 Td
(function)Tj
3.6283 0 Td
(and)Tj
1.7292 0 Td
(renal)Tj
2.245 0 Td
(disease)Tj
3.016 0 Td
([)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(].)Tj
3.0728 0 Td
(The)Tj
1.7801 0 Td
(kidneys)Tj
3.2995 0 Td
(are)Tj
1.4513 0 Td
(essential)Tj
3.583 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(urinary)Tj
3.1918 0 Td
(system)Tj
2.9423 0 Td
(and)Tj
-35.3704 -1.3039 Td
(also)Tj
1.7687 0 Td
(play)Tj
1.8709 0 Td
(a)Tj
0.652 0 Td
(role)Tj
1.7688 0 Td
(in)Tj
1.0374 0 Td
(systemic)Tj
3.6284 0 Td
(homeostasis.)Tj
5.3121 0 Td
(As)Tj
1.2642 0 Td
(urine)Tj
2.3584 0 Td
(production)Tj
4.7168 0 Td
(is)Tj
0.8448 0 Td
(regulated)Tj
3.9401 0 Td
(by)Tj
1.1792 0 Td
(oxylipins)Tj
3.8551 0 Td
(pro-)Tj
-34.1969 -1.2983 Td
(duced)Tj
2.6475 0 Td
(by)Tj
1.1849 0 Td
(the)Tj
1.4683 0 Td
(three)Tj
2.262 0 Td
(major)Tj
2.6079 0 Td
(oxygenases,)Tj
4.8925 0 Td
(which)Tj
2.6476 0 Td
(respond)Tj
3.4752 0 Td
(to)Tj
1.0262 0 Td
(systemic)Tj
3.6283 0 Td
(conditions,)Tj
4.7055 0 Td
(the)Tj
1.4683 0 Td
(urinary)Tj
3.1918 0 Td
(oxyli-)Tj
-35.206 -1.2982 Td
(pin)Tj
1.5533 0 Td
(profile)Tj
2.8403 0 Td
(may)Tj
1.9276 0 Td
(reflect)Tj
2.6929 0 Td
(renal)Tj
2.245 0 Td
(enzymatic)Tj
4.3029 0 Td
(activity)Tj
3.1181 0 Td
(as)Tj
1.0148 0 Td
(well)Tj
1.8199 0 Td
(as)Tj
1.0148 0 Td
(systemic)Tj
3.6283 0 Td
(status)Tj
2.5001 0 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(].)Tj
-27.4618 -1.2983 Td
(As)Tj
1.2699 0 Td
(an)Tj
1.2018 0 Td
(important)Tj
4.2803 0 Td
(and)Tj
1.7292 0 Td
(easily)Tj
2.4094 0 Td
(accessible)Tj
4.0875 0 Td
(biological)Tj
4.0876 0 Td
(fluid,)Tj
2.3073 0 Td
(urine)Tj
2.3585 0 Td
(has)Tj
1.542 0 Td
(been)Tj
2.1203 0 Td
(the)Tj
1.4683 0 Td
(subject)Tj
3.0217 0 Td
(of)Tj
1.0148 0 Td
(detailed)Tj
-34.0948 -1.3039 Td
(chemical)Tj
3.7813 0 Td
(analyses)Tj
3.4866 0 Td
(for)Tj
1.3833 0 Td
(more)Tj
2.3414 0 Td
(than)Tj
2.0296 0 Td
(100)Tj
1.6498 0 Td
(years.)Tj
2.4888 0 Td
(Even)Tj
2.2167 0 Td
(today,)Tj
2.6645 0 Td
(urine)Tj
2.3584 0 Td
(analysis)Tj
3.3279 0 Td
(is)Tj
0.8447 0 Td
(routinely)Tj
3.8664 0 Td
(performed)Tj
-32.4394 -1.2983 Td
(using)Tj
2.3867 0 Td
(dipstick)Tj
3.3789 0 Td
(tests)Tj
1.9615 0 Td
(that)Tj
1.7802 0 Td
(can)Tj
1.6271 0 Td
(readily)Tj
2.9423 0 Td
(measure)Tj
3.583 0 Td
(urinary)Tj
3.1918 0 Td
(glucose,)Tj
3.4015 0 Td
(bilirubin,)Tj
3.9515 0 Td
(ketone)Tj
2.9197 0 Td
(bodies,)Tj
3.0387 0 Td
(nitrates,)Tj
-34.1629 -1.2983 Td
(leukocyte)Tj
4.0194 0 Td
(esterase,)Tj
3.5433 0 Td
(specific)Tj
3.1918 0 Td
(gravity,)Tj
3.1861 0 Td
(hemoglobin,)Tj
5.2668 0 Td
(urobilinogen,)Tj
5.6295 0 Td
(and)Tj
1.7292 0 Td
(protein.)Tj
3.3788 0 Td
(More)Tj
2.4151 0 Td
(detailed)Tj
3.3676 0 Td
(uri-)Tj
-35.7276 -1.3039 Td
(nalysis)Tj
2.8913 0 Td
(can)Tj
1.627 0 Td
(be)Tj
1.1509 0 Td
(also)Tj
1.7688 0 Td
(used)Tj
2.0579 0 Td
(to)Tj
1.0262 0 Td
(study)Tj
2.3867 0 Td
(a)Tj
0.6577 0 Td
(variety)Tj
2.9196 0 Td
(of)Tj
1.0148 0 Td
(renal)Tj
2.2451 0 Td
(conditions,)Tj
4.6998 0 Td
(including)Tj
4.0421 0 Td
(bladder)Tj
3.2599 0 Td
(and)Tj
1.7291 0 Td
(kidney)Tj
-33.4769 -1.2982 Td
(cancer)Tj
2.8402 0 Td
([)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7405 0 Td
(14)Tj
0 g
(].)Tj
1.7348 0 Td
(However,)Tj
4.0762 0 Td
(some)Tj
2.33 0 Td
(problems)Tj
3.9742 0 Td
(remain)Tj
3.0841 0 Td
(to)Tj
1.0261 0 Td
(be)Tj
1.1509 0 Td
(solved)Tj
2.7382 0 Td
(in)Tj
1.0375 0 Td
(urinary)Tj
3.1974 0 Td
(lipid-based)Tj
4.6488 0 Td
(diagnos-)Tj
-33.5789 -1.2983 Td
(tics.)Tj
1.7914 0 Td
(Until)Tj
2.3187 0 Td
(recently,)Tj
3.634 0 Td
(gas)Tj
1.4797 0 Td
(chromatography)Tj
6.8825 0 Td
(coupled)Tj
3.3902 0 Td
(with)Tj
1.9956 0 Td
(MS)Tj
1.593 0 Td
(was)Tj
1.6952 0 Td
(the)Tj
1.474 0 Td
(primary)Tj
3.4525 0 Td
(lipid)Tj
2.0466 0 Td
(analysis)Tj
3.3279 0 Td
(used)Tj
-35.0813 -1.3039 Td
(for)Tj
1.3832 0 Td
(low-molecular-weight)Tj
9.0198 0 Td
(and)Tj
1.7235 0 Td
(low-polarity)Tj
5.1023 0 Td
(molecules,)Tj
4.422 0 Td
(which)Tj
2.6476 0 Td
(usually)Tj
3.0103 0 Td
(require)Tj
3.1125 0 Td
(chemical)Tj
3.7814 0 Td
(derivati-)Tj
-34.2026 -1.2983 Td
(zation.)Tj
2.9139 0 Td
(Several)Tj
3.0444 0 Td
(disease-)Tj
3.3732 0 Td
(and/or)Tj
2.9367 0 Td
(drug-)Tj
2.4605 0 Td
(related)Tj
2.948 0 Td
(metabolic)Tj
4.1442 0 Td
(abnormalities)Tj
5.7033 0 Td
(can)Tj
1.627 0 Td
(be)Tj
1.1509 0 Td
(detected)Tj
3.566 0 Td
(in)Tj
1.0374 0 Td
(urine;)Tj
-34.9055 -1.2983 Td
(however,)Tj
3.838 0 Td
(little)Tj
1.9956 0 Td
(information)Tj
5.0797 0 Td
(is)Tj
0.8447 0 Td
(available)Tj
3.668 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(urinary)Tj
3.1975 0 Td
(lipid)Tj
2.0466 0 Td
(composition)Tj
5.244 0 Td
(in)Tj
1.0318 0 Td
(healthy)Tj
3.1351 0 Td
(subjects)Tj
3.3789 0 Td
(on)Tj
-36.2038 -1.2982 Td
(a)Tj
0.652 0 Td
(regular)Tj
3.0613 0 Td
(diet,)Tj
1.9616 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(normal)Tj
3.1465 0 Td
(variation)Tj
3.804 0 Td
(in)Tj
1.0375 0 Td
(urinary)Tj
3.1918 0 Td
(lipid)Tj
2.0466 0 Td
(content)Tj
3.2655 0 Td
(has)Tj
1.5477 0 Td
(not)Tj
1.5704 0 Td
(been)Tj
2.1259 0 Td
(determined,)Tj
-30.6082 -1.3039 Td
(which)Tj
2.6475 0 Td
(restricts)Tj
3.3959 0 Td
(the)Tj
1.4683 0 Td
(diagnostic)Tj
4.32 0 Td
(potential)Tj
3.7644 0 Td
(of)Tj
1.0148 0 Td
(lipid)Tj
2.0409 0 Td
(profiling)Tj
3.702 0 Td
(in)Tj
1.0318 0 Td
(urine)Tj
2.3584 0 Td
(samples)Tj
3.3846 0 Td
(despite)Tj
3.0387 0 Td
(its)Tj
1.1452 0 Td
(clinical)Tj
-33.3125 -1.2983 Td
(significance.)Tj
1.1962 -1.2983 Td
(To)Tj
1.3379 0 Td
(overcome)Tj
4.1499 0 Td
(these)Tj
2.2564 0 Td
(obstacles,)Tj
4.0081 0 Td
(we)Tj
1.3266 0 Td
(have)Tj
2.058 0 Td
(attempted)Tj
4.2632 0 Td
(to)Tj
1.0262 0 Td
(characterize)Tj
5.0286 0 Td
(the)Tj
1.4683 0 Td
(human)Tj
3.0785 0 Td
(urine)Tj
2.3584 0 Td
(metabo-)Tj
-33.5563 -1.3039 Td
(lome)Tj
2.2166 0 Td
(in)Tj
1.0318 0 Td
(healthy)Tj
3.1351 0 Td
(subjects.)Tj
3.6057 0 Td
(In)Tj
1.1055 0 Td
(this)Tj
1.6724 0 Td
(study,)Tj
2.6192 0 Td
(we)Tj
1.3266 0 Td
(performed)Tj
4.4674 0 Td
(cross-classification)Tj
7.7215 0 Td
(of)Tj
1.0148 0 Td
(urinary)Tj
3.1975 0 Td
(lipids)Tj
2.4037 0 Td
(by)Tj
-35.5178 -1.2982 Td
(sex,)Tj
1.695 0 Td
(age,)Tj
1.7689 0 Td
(and)Tj
1.7291 0 Td
(body)Tj
2.2167 0 Td
(mass)Tj
2.194 0 Td
(index)Tj
2.4547 0 Td
(\(BMI\))Tj
2.7099 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
0.652 0 Td
(healthy)Tj
3.1351 0 Td
(population)Tj
4.5864 0 Td
(after)Tj
2.0353 0 Td
(normal)Tj
3.1464 0 Td
(overnight)Tj
4.0875 0 Td
(fasting)Tj
-33.4485 -1.2983 Td
(using)Tj
2.3867 0 Td
(liquid)Tj
2.5625 0 Td
(chromatography)Tj
6.8825 0 Td
(coupled)Tj
3.3902 0 Td
(with)Tj
1.9956 0 Td
(MS)Tj
1.593 0 Td
(\(LC/MS\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 247.9748 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Materials)Tj
4.5071 0 Td
(and)Tj
1.989 0 Td
(Methods)Tj
/F0 1 Tf
-6.4961 -1.4976 Td
(Collection)Tj
4.5449 0 Td
(and)Tj
1.8661 0 Td
(preparation)Tj
5.2111 0 Td
(of)Tj
1.0441 0 Td
(human)Tj
3.2457 0 Td
(urine)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 212.9952 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Urine)Tj
2.5681 0 Td
(samples)Tj
3.3789 0 Td
(from)Tj
2.2053 0 Td
(healthy)Tj
3.1351 0 Td
(white)Tj
2.4208 0 Td
(adults)Tj
2.6135 0 Td
(were)Tj
2.1203 0 Td
(purchased)Tj
4.337 0 Td
(from)Tj
2.2054 0 Td
(PromedDX)Tj
4.8188 0 Td
(\(Norton,)Tj
3.7531 0 Td
(MA\).)Tj
2.3641 0 Td
(The)Tj
-35.9204 -1.2982 Td
(samples)Tj
3.3788 0 Td
(were)Tj
2.126 0 Td
(collected)Tj
3.736 0 Td
(after)Tj
2.0353 0 Td
(written)Tj
3.1124 0 Td
(informed)Tj
3.9742 0 Td
(consent)Tj
3.3278 0 Td
(was)Tj
1.7008 0 Td
(obtained)Tj
3.736 0 Td
(from)Tj
2.2054 0 Td
(all)Tj
1.1452 0 Td
(subjects.)Tj
3.6056 0 Td
(These)Tj
-34.0835 -1.3039 Td
(were)Tj
2.1203 0 Td
(collected)Tj
3.7417 0 Td
(using)Tj
2.3867 0 Td
(the)Tj
1.4684 0 Td
(blood)Tj
2.5171 0 Td
(donor)Tj
2.6816 0 Td
(history)Tj
3.0047 0 Td
(questionnaires)Tj
6.0661 0 Td
(prepared)Tj
3.8097 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.4684 0 Td
(AABB)Tj
2.7495 0 Td
(to)Tj
1.0262 0 Td
(screen)Tj
-34.2196 -1.2983 Td
(for)Tj
1.3832 0 Td
(disease)Tj
3.0218 0 Td
(risk)Tj
1.7121 0 Td
(factors)Tj
2.9026 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(].)Tj
2.0807 0 Td
(Therefore,)Tj
4.388 0 Td
(all)Tj
1.1508 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
2.2507 0 Td
(subjects)Tj
3.3789 0 Td
(are)Tj
1.4513 0 Td
(considered)Tj
4.5978 0 Td
(as)Tj
1.0148 0 Td
(Normal)Tj
3.3335 0 Td
(Healthy)Tj
-33.681 -1.2983 Td
(donors)Tj
3.0387 0 Td
(outlined)Tj
3.5659 0 Td
(by)Tj
1.1849 0 Td
(AABB.)Tj
2.9707 0 Td
(The)Tj
1.7858 0 Td
(ethics)Tj
2.5172 0 Td
(committee)Tj
4.5014 0 Td
(of)Tj
1.0147 0 Td
(the)Tj
1.4741 0 Td
(National)Tj
3.6906 0 Td
(Institute)Tj
3.583 0 Td
(of)Tj
1.0148 0 Td
(Health)Tj
2.914 0 Td
(Sciences)Tj
-33.2558 -1.2982 Td
(authorized)Tj
4.5183 0 Td
(PromedDX)Tj
4.8132 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.6577 0 Td
(validated)Tj
3.8324 0 Td
(provider)Tj
3.6567 0 Td
(of)Tj
1.0148 0 Td
(urine)Tj
2.3584 0 Td
(samples)Tj
3.3845 0 Td
(and)Tj
1.7291 0 Td
(exempted)Tj
4.1216 0 Td
(us)Tj
1.1055 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
-34.4123 -1.3039 Td
(committee's)Tj
5.0739 0 Td
(approval)Tj
3.7021 0 Td
(for)Tj
1.3889 0 Td
(use)Tj
1.5307 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(purchased)Tj
4.3369 0 Td
(urine)Tj
2.3584 0 Td
(samples.)Tj
3.6057 0 Td
(All)Tj
1.3946 0 Td
(subjects)Tj
3.3789 0 Td
(declared)Tj
3.6 0 Td
(healthy)Tj
3.1294 0 Td
(at)Tj
-35.9827 -1.2983 Td
(the)Tj
1.4683 0 Td
(recruitment,)Tj
5.2157 0 Td
(and)Tj
1.7348 0 Td
(their)Tj
2.109 0 Td
(urine)Tj
2.3584 0 Td
(was)Tj
1.6951 0 Td
(collected)Tj
3.7417 0 Td
(the)Tj
1.4683 0 Td
(next)Tj
1.9559 0 Td
(morning)Tj
3.7474 0 Td
(after)Tj
2.0353 0 Td
(fasting)Tj
2.8799 0 Td
(for)Tj
1.389 0 Td
(14)Tj
1.1679 0 Td
(h)Tj
0.7483 0 Td
(from)Tj
2.2054 0 Td
(60)Tj
-35.9204 -1.2983 Td
(individuals)Tj
4.6487 0 Td
(divided)Tj
3.2145 0 Td
(into)Tj
1.8425 0 Td
(four)Tj
1.9106 0 Td
(groups)Tj
2.9706 0 Td
(\(n)Tj
1.1112 0 Td
(=)Tj
0.7994 0 Td
(15\):)Tj
1.7348 0 Td
(young)Tj
2.7212 0 Td
(men)Tj
2.0126 0 Td
(\(2533)Tj
2.982 0 Td
(years)Tj
2.2621 0 Td
(old\),)Tj
2.0692 0 Td
(elderly)Tj
2.914 0 Td
(men)Tj
2.0126 0 Td
(\(55)Tj
-35.206 -1.3039 Td
(64)Tj
1.1678 0 Td
(years)Tj
2.2677 0 Td
(old\),)Tj
2.0636 0 Td
(young)Tj
2.7269 0 Td
(women)Tj
3.2032 0 Td
(\(2534)Tj
2.9763 0 Td
(years)Tj
2.2677 0 Td
(old\),)Tj
2.0636 0 Td
(and)Tj
1.7292 0 Td
(elderly)Tj
2.914 0 Td
(women)Tj
3.2031 0 Td
(\(5563)Tj
2.982 0 Td
(years)Tj
2.2621 0 Td
(old\))Tj
1.8425 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Table)Tj
2.7836 0 Td
(1)Tj
0 g
-36.4533 -1.2983 Td
(and)Tj
0.83 0.64 0.02 0 k
1.7291 0 Td
(S1)Tj
1.1735 0 Td
(Table)Tj
0 g
(\).)Tj
3.0047 0 Td
(Fresh)Tj
2.4321 0 Td
(urine)Tj
2.3584 0 Td
(from)Tj
2.2054 0 Td
(each)Tj
2.0296 0 Td
(individual)Tj
4.2859 0 Td
(was)Tj
1.7008 0 Td
(collected)Tj
3.736 0 Td
(into)Tj
1.8426 0 Td
(15-ml)Tj
2.5908 0 Td
(test)Tj
1.5987 0 Td
(tubes,)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 442.0346 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Urinary)Tj
3.3803 0 Td
(Lipidome)Tj
4.2024 0 Td
(in)Tj
0.9779 0 Td
(Healthy)Tj
3.5505 0 Td
(Popula)Tj
3.0472 0 Td
(tion)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.4748 0 Td
(DOI:10.137)Tj
5.0387 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.0168)Tj
3.5434 0 Td
(188)Tj
2.6362 0 Td
(December)Tj
4.7622 0 Td
(14,)Tj
1.5874 0 Td
(2016)Tj
36.7515 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(13)Tj
/F7 1 Tf
-0.025 Tc
-66.402 84.0904 Td
(Competing)Tj
4.3795 0 Td
(Interests)Tj
3.3307 0 Td
(:)Tj
/F8 1 Tf
0.4819 0 Td
(The)Tj
1.5875 0 Td
(authors)Tj
3.0401 0 Td
(have)Tj
1.963 0 Td
(declared)Tj
-14.7827 -1.3748 Td
(that)Tj
1.6228 0 Td
(no)Tj
1.1693 0 Td
(competing)Tj
4.1599 0 Td
(interests)Tj
ET
endstream
endobj
28 0 obj
<>stream
endstream
endobj
29 0 obj
<>stream
endstream
endobj
30 0 obj
<>stream
endstream
endobj
31 0 obj
<>stream
endstream
endobj
32 0 obj
<>stream
endstream
endobj
33 0 obj
<>stream
endstream
endobj
34 0 obj
<>stream
endstream
endobj
35 0 obj
<>stream
endstream
endobj
36 0 obj
<>stream
endstream
endobj
37 0 obj
<>stream
endstream
endobj
38 0 obj
<>stream
endstream
endobj
39 0 obj
<>stream
endstream
endobj
40 0 obj
<>stream
endstream
endobj
41 0 obj
<>stream
endstream
endobj
42 0 obj
<>stream
BT
10.3394 82.7156 Td
(exist.)Tj
ET
Q
endstream
endobj
43 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 11 0 R/Annots 47 0 R/Contents 48 0 R/TrimBox[0 0 612 792]>>
endobj
47 0 obj
[49 0 R 50 0 R 51 0 R 52 0 R 53 0 R]
endobj
49 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref016)>>
endobj
50 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref017)>>
endobj
51 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref017)>>
endobj
52 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref016)>>
endobj
53 0 obj
<>/Border[0 0 0]/Dest(Lpone.0168188.t001)>>
endobj
48 0 obj
[54 0 R 55 0 R 56 0 R 57 0 R 58 0 R 59 0 R]
endobj
54 0 obj
<>stream
q
0.83 0.64 0.02 0 k
244.4031 386.6457 m
253.9276 386.6457 l
h
f*
258.3496 386.6457 m
267.874 386.6457 l
h
f*
489.0331 386.6457 m
498.5575 386.6457 l
h
f*
258.1795 81.9213 m
267.6472 81.9213 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 439.7102 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(immediately)Tj
5.1987 0 Td
(frozen,)Tj
2.999 0 Td
(and)Tj
1.7291 0 Td
(stored)Tj
2.7099 0 Td
(at)Tj
0.9525 0 Td
(-80C.)Tj
2.8006 0 Td
(The)Tj
1.7858 0 Td
(samples)Tj
3.3789 0 Td
(were)Tj
2.1259 0 Td
(shipped)Tj
3.3619 0 Td
(from)Tj
2.2053 0 Td
(PromedDX)Tj
4.8132 0 Td
(on)Tj
1.2756 0 Td
(dry)Tj
-35.3364 -1.2982 Td
(ice,)Tj
1.559 0 Td
(thawed)Tj
3.1124 0 Td
(on)Tj
1.2756 0 Td
(ice)Tj
1.3379 0 Td
(after)Tj
2.0353 0 Td
(arrival,)Tj
3.0217 0 Td
(divided)Tj
3.2145 0 Td
(into)Tj
1.8425 0 Td
(small)Tj
2.3244 0 Td
(aliquots,)Tj
3.5943 0 Td
(and)Tj
1.7348 0 Td
(stored)Tj
2.7042 0 Td
(at)Tj
0.9525 0 Td
(-80C)Tj
2.5795 0 Td
(until)Tj
2.1089 0 Td
(lipid)Tj
-33.3975 -1.3039 Td
(extraction.)Tj
4.4787 0 Td
(Together)Tj
3.8494 0 Td
(with)Tj
1.9956 0 Td
(urine,)Tj
2.5851 0 Td
(fresh)Tj
2.194 0 Td
(blood)Tj
2.5115 0 Td
(was)Tj
1.7008 0 Td
(simultaneously)Tj
6.2419 0 Td
(collected)Tj
3.7417 0 Td
(from)Tj
2.2053 0 Td
(same)Tj
2.2564 0 Td
(individu-)Tj
-33.7604 -1.2983 Td
(als,)Tj
1.4853 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4684 0 Td
(metabolites)Tj
4.8075 0 Td
(profiles)Tj
3.2031 0 Td
(in)Tj
1.0318 0 Td
(their)Tj
2.109 0 Td
(plasma)Tj
3.033 0 Td
(and)Tj
1.7292 0 Td
(serum)Tj
2.7269 0 Td
(are)Tj
1.4456 0 Td
(already)Tj
3.1124 0 Td
(published)Tj
4.1273 0 Td
(in)Tj
1.0318 0 Td
(our)Tj
1.627 0 Td
(previ-)Tj
-34.6674 -1.2983 Td
(ous)Tj
1.6157 0 Td
(paper)Tj
2.4831 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(17)Tj
0 g
(].)Tj
1.7348 0 Td
(There)Tj
2.5795 0 Td
(are)Tj
1.4513 0 Td
(no)Tj
1.2756 0 Td
(outliers)Tj
3.2202 0 Td
(in)Tj
1.0374 0 Td
(their)Tj
2.1033 0 Td
(serum)Tj
2.7269 0 Td
(creatinine)Tj
4.2236 0 Td
(levels)Tj
2.3755 0 Td
([)Tj
0.83 0.64 0.02 0 k
(17)Tj
0 g
(],)Tj
2.0749 0 Td
(a)Tj
0.6576 0 Td
(classical)Tj
-31.2942 -1.2982 Td
(biomarker)Tj
4.4163 0 Td
(for)Tj
1.3833 0 Td
(kidney)Tj
2.9423 0 Td
(function,)Tj
3.8551 0 Td
(suggesting)Tj
4.3994 0 Td
(their)Tj
2.1089 0 Td
(renal)Tj
2.2451 0 Td
(functions)Tj
3.9855 0 Td
(are)Tj
1.4513 0 Td
(normal.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 343.3322 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Chemicals)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 326.3811 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Unless)Tj
2.8969 0 Td
(otherwise)Tj
4.0819 0 Td
(indicated,)Tj
4.1612 0 Td
(all)Tj
1.1452 0 Td
(reagents)Tj
3.549 0 Td
(were)Tj
2.1203 0 Td
(of)Tj
1.0148 0 Td
(MS)Tj
1.5874 0 Td
(analytical)Tj
4.0081 0 Td
(grade.)Tj
2.6703 0 Td
(LC/MS)Tj
3.118 0 Td
(grade)Tj
2.4435 0 Td
(methanol,)Tj
-32.7966 -1.3039 Td
(acetonitrile,)Tj
4.9549 0 Td
(water,)Tj
2.6532 0 Td
(and)Tj
1.7291 0 Td
(chloroform)Tj
4.7905 0 Td
(were)Tj
2.1203 0 Td
(purchased)Tj
4.337 0 Td
(from)Tj
2.2054 0 Td
(Wako)Tj
2.6248 0 Td
(Pure)Tj
2.0976 0 Td
(Chemical,)Tj
4.252 0 Td
(Ltd.)Tj
1.8085 0 Td
(\(Nihon-)Tj
-33.5733 -1.2982 Td
(bashi,)Tj
2.5398 0 Td
(Tokyo\).)Tj
3.3618 0 Td
(1,2-dipalmitoyl)Tj
6.3326 0 Td
(d6-3-sn)Tj
3.3108 0 Td
(glycerophosphatidylcholine)Tj
11.2422 0 Td
(\(16:0/16:0)Tj
4.1896 0 Td
(PC-d6\))Tj
3.1407 0 Td
(and)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 558.4818 304.7811 cm
BT
/F5 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(13)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 565.5685 300.359 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(C-)Tj
-36.5553 -1.2982 Td
(labeled)Tj
3.0274 0 Td
(tripalmitin)Tj
4.5524 0 Td
(\(tripalmitin-1,1,1-)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 348.3779 291.7984 cm
BT
/F5 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(13)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 355.4645 287.3763 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(C)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 362.0976 285.6755 cm
BT
/F5 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(3)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 365.6692 287.3763 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\))Tj
0.5612 0 Td
(were)Tj
2.1203 0 Td
(purchased)Tj
4.337 0 Td
(from)Tj
2.2053 0 Td
(Larodan)Tj
3.5773 0 Td
(Malmo)Tj
3.1238 0 Td
(\(Solna,)Tj
2.9934 0 Td
(Swe-)Tj
-35.4838 -1.3039 Td
(den\).)Tj
2.2847 0 Td
(Phospholipid,)Tj
5.777 0 Td
(PUFA,)Tj
2.9536 0 Td
(and)Tj
1.7292 0 Td
(other)Tj
2.347 0 Td
(oxylipin)Tj
3.4922 0 Td
(internal)Tj
3.3619 0 Td
(standards)Tj
4.0818 0 Td
(\(ISs\))Tj
2.075 0 Td
(were)Tj
2.126 0 Td
(purchased)Tj
4.3313 0 Td
(from)Tj
-34.5597 -1.2982 Td
(Cayman)Tj
3.5716 0 Td
(\(Ann)Tj
2.3414 0 Td
(Arbor,)Tj
2.8857 0 Td
(MI\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 230.0031 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Sample)Tj
3.5716 0 Td
(preparation)Tj
5.2111 0 Td
(for)Tj
1.3748 0 Td
(MS)Tj
1.7103 0 Td
(analysis)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 212.9952 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Urine)Tj
2.5681 0 Td
(\(200)Tj
/F10 1 Tf
1.9843 0 Td
[()]TJ
/F5 1 Tf
(l\))Tj
1.3152 0 Td
(was)Tj
1.6951 0 Td
(added)Tj
2.6646 0 Td
(to)Tj
1.0205 0 Td
(1,300)Tj
/F10 1 Tf
2.3527 0 Td
[()]TJ
/F5 1 Tf
(l)Tj
0.9694 0 Td
(of)Tj
1.0148 0 Td
(methanol)Tj
4.0252 0 Td
(containing)Tj
4.5297 0 Td
(ISs)Tj
1.3947 0 Td
(used)Tj
2.0636 0 Td
(for)Tj
1.3833 0 Td
(normalization)Tj
5.8847 0 Td
(and)Tj
-34.8659 -1.2982 Td
(evaluation)Tj
4.3596 0 Td
(of)Tj
1.0148 0 Td
(extraction)Tj
4.2576 0 Td
(efficacy.)Tj
3.4469 0 Td
(The)Tj
1.7802 0 Td
(following)Tj
3.9798 0 Td
(ISs)Tj
1.4003 0 Td
(were)Tj
2.1203 0 Td
(utilized:)Tj
3.4186 0 Td
(16:0/16:0)Tj
3.8494 0 Td
(PC-d6)Tj
2.8006 0 Td
(\(33.3)Tj
/F10 1 Tf
2.211 0 Td
[()]TJ
/F5 1 Tf
(M\),)Tj
-34.6391 -1.3039 Td
(tripalmitin-1,1,1-)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 269.1212 191.4519 cm
BT
/F5 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(13)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 276.2645 186.9732 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(C)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 282.8976 185.3291 cm
BT
/F5 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(3)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 288.6236 186.9732 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\(3.3)Tj
/F10 1 Tf
1.7404 0 Td
[()]TJ
/F5 1 Tf
(M\),)Tj
2.1827 0 Td
(deuterated)Tj
4.4787 0 Td
(PGE)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 391.0677 185.3291 cm
BT
/F5 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 394.6393 186.9732 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(-d4)Tj
1.5703 0 Td
(\(33)Tj
1.5137 0 Td
(pg/)Tj
/F10 1 Tf
[()]TJ
/F5 1 Tf
(l\),)Tj
2.8517 0 Td
(and)Tj
1.7291 0 Td
(deuterated)Tj
4.473 0 Td
(LTB)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 533.3102 185.3291 cm
BT
/F5 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(4)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 536.8818 186.9732 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(-d4)Tj
1.576 0 Td
(\(33)Tj
-35.2627 -1.2982 Td
(pg/)Tj
/F10 1 Tf
[()]TJ
/F5 1 Tf
(l\).)Tj
2.8517 0 Td
(The)Tj
1.7801 0 Td
(samples)Tj
3.3846 0 Td
(were)Tj
2.1203 0 Td
(centrifuged)Tj
4.7848 0 Td
(at)Tj
0.9524 0 Td
(15,000)Tj
2.8233 0 Td
(rpm)Tj
1.9276 0 Td
(for)Tj
1.389 0 Td
(3)Tj
0.6916 0 Td
(min)Tj
1.8595 0 Td
(at)Tj
0.9525 0 Td
(4C)Tj
1.7461 0 Td
(to)Tj
1.0261 0 Td
(precipitate)Tj
4.4617 0 Td
(insoluble)Tj
-32.7513 -1.2983 Td
(matter,)Tj
3.0898 0 Td
(and)Tj
1.7291 0 Td
(1,440)Tj
/F10 1 Tf
2.3471 0 Td
[()]TJ
/F5 1 Tf
(l)Tj
0.9751 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(supernatant)Tj
4.9833 0 Td
(was)Tj
1.7008 0 Td
(diluted)Tj
3.0387 0 Td
(9.9-fold)Tj
3.3165 0 Td
(using)Tj
2.3868 0 Td
(Milli-Q)Tj
3.2371 0 Td
(water)Tj
2.4321 0 Td
(adjusted)Tj
3.5772 0 Td
(to)Tj
-35.2967 -1.2982 Td
(pH)Tj
1.5024 0 Td
(3.0)Tj
1.4003 0 Td
(with)Tj
1.9956 0 Td
(1N)Tj
1.4286 0 Td
(HCl)Tj
1.8992 0 Td
(and)Tj
1.7291 0 Td
(subjected)Tj
3.9685 0 Td
(to)Tj
1.0262 0 Td
(solid-phase)Tj
4.7451 0 Td
(extraction)Tj
4.2576 0 Td
(using)Tj
2.3868 0 Td
(Sep-Pak)Tj
3.4752 0 Td
(Vac.)Tj
2.0013 0 Td
(RC)Tj
1.5023 0 Td
(\(500-mg\))Tj
-33.3182 -1.304 Td
(C18)Tj
1.8312 0 Td
(cartridges)Tj
4.1669 0 Td
(\(Waters,)Tj
3.6397 0 Td
(Milford,)Tj
3.5433 0 Td
(MA\).)Tj
2.364 0 Td
(The)Tj
1.7802 0 Td
(samples)Tj
3.3845 0 Td
(were)Tj
2.1203 0 Td
(then)Tj
2.0183 0 Td
(eluted)Tj
2.6702 0 Td
(sequentially)Tj
4.9549 0 Td
(with)Tj
1.9955 0 Td
(methyl)Tj
-34.469 -1.2982 Td
(formate)Tj
3.3619 0 Td
(and)Tj
1.7291 0 Td
(methanol.)Tj
4.252 0 Td
(Lipid)Tj
2.3357 0 Td
(metabolites)Tj
4.8075 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.4684 0 Td
(methanol)Tj
4.0251 0 Td
(fractions)Tj
3.7191 0 Td
(were)Tj
2.1259 0 Td
(measured)Tj
4.1046 0 Td
(by)Tj
1.1848 0 Td
(ultra-)Tj
-34.1459 -1.2983 Td
(performance)Tj
5.3461 0 Td
(liquid)Tj
2.5625 0 Td
(chromatography/time-)Tj
9.1502 0 Td
(of-flight)Tj
3.4583 0 Td
(mass)Tj
2.194 0 Td
(spectrometry)Tj
5.4878 0 Td
(\(UPLC-TOFMS;)Tj
6.8881 0 Td
(LCT)Tj
-35.087 -1.3039 Td
(Premier)Tj
3.4526 0 Td
(XE;)Tj
1.6668 0 Td
(Waters)Tj
3.0784 0 Td
(Micro-mass;)Tj
5.2327 0 Td
(Waters\))Tj
3.4129 0 Td
(for)Tj
1.3833 0 Td
(analysis)Tj
3.3278 0 Td
(of)Tj
1.0148 0 Td
(phospholipids,)Tj
6.0945 0 Td
(sphingolipids,)Tj
5.8279 0 Td
(and)Tj
-34.4917 -1.2983 Td
(neutral)Tj
3.0671 0 Td
(lipids)Tj
2.4094 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(].)Tj
2.075 0 Td
(PUFAs)Tj
3.0897 0 Td
(and)Tj
1.7291 0 Td
(their)Tj
2.109 0 Td
(oxidation)Tj
4.0422 0 Td
(products)Tj
3.753 0 Td
(\(oxylipins\))Tj
4.5354 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.4741 0 Td
(methyl)Tj
2.9877 0 Td
(formate)Tj
ET
Q
q
0.45 0.36 0.35 0.01 k
1 j
1 J
0 w
199.9998 703.6809 m
575.9997 703.6809 l
575.9997 703.1809 l
199.9998 703.1809 l
f*
372.5334 703.1809 m
372.5334 690.1809 l
372.0334 690.681 l
372.0334 703.1809 l
f*
372.5334 690.1809 m
199.9998 690.1809 l
199.9998 690.681 l
372.0334 690.681 l
f*
476.718 703.1809 m
476.718 690.1809 l
476.2179 690.681 l
476.2179 703.1809 l
f*
476.718 690.1809 m
372.5334 690.1809 l
372.5334 690.681 l
476.2179 690.681 l
f*
575.9997 690.1809 m
476.718 690.1809 l
476.718 690.681 l
575.9997 690.681 l
f*
372.5334 690.1809 m
372.5334 677.681 l
372.0334 678.181 l
372.0334 690.1809 l
f*
372.5334 677.681 m
199.9998 677.681 l
199.9998 678.181 l
372.0334 678.181 l
f*
476.718 690.1809 m
476.718 677.681 l
476.2179 678.181 l
476.2179 690.1809 l
f*
476.718 677.681 m
372.5334 677.681 l
372.5334 678.181 l
476.2179 678.181 l
f*
575.9997 677.681 m
476.718 677.681 l
476.718 678.181 l
575.9997 678.181 l
f*
372.5334 677.681 m
372.5334 665.1811 l
372.0334 665.6811 l
372.0334 677.681 l
f*
372.5334 665.1811 m
199.9998 665.1811 l
199.9998 665.6811 l
372.0334 665.6811 l
f*
476.718 677.681 m
476.718 665.1811 l
476.2179 665.6811 l
476.2179 677.681 l
f*
476.718 665.1811 m
372.5334 665.1811 l
372.5334 665.6811 l
476.2179 665.6811 l
f*
575.9997 665.1811 m
476.718 665.1811 l
476.718 665.6811 l
575.9997 665.6811 l
f*
372.5334 665.1811 m
372.5334 652.6811 l
372.0334 653.1812 l
372.0334 665.1811 l
f*
372.5334 652.6811 m
199.9998 652.6811 l
199.9998 653.1812 l
372.0334 653.1812 l
f*
476.718 665.1811 m
476.718 652.6811 l
476.2179 653.1812 l
476.2179 665.1811 l
f*
476.718 652.6811 m
372.5334 652.6811 l
372.5334 653.1812 l
476.2179 653.1812 l
f*
575.9997 652.6811 m
476.718 652.6811 l
476.718 653.1812 l
575.9997 653.1812 l
f*
372.5334 652.6811 m
372.5334 640.1812 l
372.0334 640.6812 l
372.0334 652.6811 l
f*
372.5334 640.1812 m
199.9998 640.1812 l
199.9998 640.6812 l
372.0334 640.6812 l
f*
476.718 652.6811 m
476.718 640.1812 l
476.2179 640.6812 l
476.2179 652.6811 l
f*
476.718 640.1812 m
372.5334 640.1812 l
372.5334 640.6812 l
476.2179 640.6812 l
f*
575.9997 640.1812 m
476.718 640.1812 l
476.718 640.6812 l
575.9997 640.6812 l
f*
372.5334 640.1812 m
372.5334 627.6813 l
372.0334 628.1813 l
372.0334 640.1812 l
f*
372.5334 627.6813 m
199.9998 627.6813 l
199.9998 628.1813 l
372.0334 628.1813 l
f*
476.718 640.1812 m
476.718 627.6813 l
476.2179 628.1813 l
476.2179 640.1812 l
f*
476.718 627.6813 m
372.5334 627.6813 l
372.5334 628.1813 l
476.2179 628.1813 l
f*
575.9997 627.6813 m
476.718 627.6813 l
476.718 628.1813 l
575.9997 628.1813 l
f*
372.5334 627.6813 m
372.5334 615.1813 l
372.0334 615.6814 l
372.0334 627.6813 l
f*
372.5334 615.1813 m
199.9998 615.1813 l
199.9998 615.6814 l
372.0334 615.6814 l
f*
476.718 627.6813 m
476.718 615.1813 l
476.2179 615.6814 l
476.2179 627.6813 l
f*
476.718 615.1813 m
372.5334 615.1813 l
372.5334 615.6814 l
476.2179 615.6814 l
f*
575.9997 615.1813 m
476.718 615.1813 l
476.718 615.6814 l
575.9997 615.6814 l
f*
372.5334 615.1813 m
372.5334 602.6814 l
372.0334 603.1814 l
372.0334 615.1813 l
f*
372.5334 602.6814 m
199.9998 602.6814 l
199.9998 603.1814 l
372.0334 603.1814 l
f*
476.718 615.1813 m
476.718 602.6814 l
476.2179 603.1814 l
476.2179 615.1813 l
f*
476.718 602.6814 m
372.5334 602.6814 l
372.5334 603.1814 l
476.2179 603.1814 l
f*
575.9997 602.6814 m
476.718 602.6814 l
476.718 603.1814 l
575.9997 603.1814 l
f*
372.5334 602.6814 m
372.5334 590.1814 l
372.0334 590.6815 l
372.0334 602.6814 l
f*
372.5334 590.1814 m
199.9998 590.1814 l
199.9998 590.6815 l
372.0334 590.6815 l
f*
476.718 602.6814 m
476.718 590.1814 l
476.2179 590.6815 l
476.2179 602.6814 l
f*
476.718 590.1814 m
372.5334 590.1814 l
372.5334 590.6815 l
476.2179 590.6815 l
f*
575.9997 590.1814 m
476.718 590.1814 l
476.718 590.6815 l
575.9997 590.6815 l
f*
372.5334 590.1814 m
372.5334 577.6815 l
372.0334 578.1815 l
372.0334 590.1814 l
f*
372.5334 577.6815 m
199.9998 577.6815 l
199.9998 578.1815 l
372.0334 578.1815 l
f*
476.718 590.1814 m
476.718 577.6815 l
476.2179 578.1815 l
476.2179 590.1814 l
f*
476.718 577.6815 m
372.5334 577.6815 l
372.5334 578.1815 l
476.2179 578.1815 l
f*
575.9997 577.6815 m
476.718 577.6815 l
476.718 578.1815 l
575.9997 578.1815 l
f*
372.5334 577.6815 m
372.5334 565.1816 l
372.0334 565.6816 l
372.0334 577.6815 l
f*
372.5334 565.1816 m
199.9998 565.1816 l
199.9998 565.6816 l
372.0334 565.6816 l
f*
476.718 577.6815 m
476.718 565.1816 l
476.2179 565.6816 l
476.2179 577.6815 l
f*
476.718 565.1816 m
372.5334 565.1816 l
372.5334 565.6816 l
476.2179 565.6816 l
f*
575.9997 565.1816 m
476.718 565.1816 l
476.718 565.6816 l
575.9997 565.6816 l
f*
372.5334 565.1816 m
372.5334 552.6816 l
372.0334 553.1817 l
372.0334 565.1816 l
f*
372.5334 552.6816 m
199.9998 552.6816 l
199.9998 553.1817 l
372.0334 553.1817 l
f*
476.718 565.1816 m
476.718 552.6816 l
476.2179 553.1817 l
476.2179 565.1816 l
f*
476.718 552.6816 m
372.5334 552.6816 l
372.5334 553.1817 l
476.2179 553.1817 l
f*
575.9997 552.6816 m
476.718 552.6816 l
476.718 553.1817 l
575.9997 553.1817 l
f*
372.5334 552.6816 m
372.5334 540.1817 l
372.0334 540.6817 l
372.0334 552.6816 l
f*
372.5334 540.1817 m
199.9998 540.1817 l
199.9998 540.6817 l
372.0334 540.6817 l
f*
476.718 552.6816 m
476.718 540.1817 l
476.2179 540.6817 l
476.2179 552.6816 l
f*
476.718 540.1817 m
372.5334 540.1817 l
372.5334 540.6817 l
476.2179 540.6817 l
f*
575.9997 540.1817 m
476.718 540.1817 l
476.718 540.6817 l
575.9997 540.6817 l
f*
372.5334 540.1817 m
372.5334 527.6818 l
372.0334 528.1818 l
372.0334 540.1817 l
f*
372.5334 527.6818 m
199.9998 527.6818 l
199.9998 528.1818 l
372.0334 528.1818 l
f*
476.718 540.1817 m
476.718 527.6818 l
476.2179 528.1818 l
476.2179 540.1817 l
f*
476.718 527.6818 m
372.5334 527.6818 l
372.5334 528.1818 l
476.2179 528.1818 l
f*
575.9997 527.6818 m
476.718 527.6818 l
476.718 528.1818 l
575.9997 528.1818 l
f*
575.9997 481.9317 m
199.9998 481.9317 l
199.9998 482.4317 l
575.9997 482.4317 l
f*
0 g
7.9999 0 0 7.9999 200.0125 708.4913 cm
BT
/F2 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Table)Tj
2.7992 0 Td
(1.)Tj
1.2614 0 Td
(Demogra)Tj
4.2733 0 Td
(phic)Tj
2.2535 0 Td
(characterist)Tj
5.5702 0 Td
(ics)Tj
1.5874 0 Td
(of)Tj
1.1551 0 Td
(the)Tj
1.7008 0 Td
(study)Tj
2.8488 0 Td
(population.)Tj
0 Tc
-23.4497 -1.8496 Td
(GROUP)Tj
3.8834 0 Td
(\(numbe)Tj
3.6001 0 Td
(r\))Tj
14.3292 0 Td
(AGE)Tj
/F11 1 Tf
[( )]TJ
/F2 1 Tf
(Std)Tj
13.0253 0 Td
(BMI)Tj
/F11 1 Tf
[( )]TJ
/F2 1 Tf
(Std)Tj
/F0 1 Tf
-34.838 -1.6299 Td
(YOUNG)Tj
3.8834 0 Td
(MEN)Tj
2.4449 0 Td
(\(n)Tj
1.1127 0 Td
(=)Tj
0.8078 0 Td
(15\))Tj
13.6915 0 Td
(29.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(2.4)Tj
13.0253 0 Td
(26.2)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(5.6)Tj
-34.9656 -1.5591 Td
(ELDERLY)Tj
4.7551 0 Td
(MEN)Tj
2.4449 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8079 0 Td
(15\))Tj
12.8198 0 Td
(59.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(2.57)Tj
13.0253 0 Td
(24.5)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(4.7)Tj
-34.9656 -1.5661 Td
(MEN)Tj
2.4378 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8149 0 Td
(30\))Tj
17.575 0 Td
(44.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(15.1)Tj
13.0253 0 Td
(25.1)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(5.2)Tj
-34.9656 -1.5591 Td
(YOUNG)Tj
3.8834 0 Td
(WOMEN)Tj
4.167 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8079 0 Td
(15\))Tj
11.9694 0 Td
(28.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(3.3)Tj
13.0253 0 Td
(35.4)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(8.8)Tj
-34.9656 -1.5591 Td
(ELDERLY)Tj
4.7551 0 Td
(WOMEN)Tj
4.167 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8079 0 Td
(15\))Tj
11.0977 0 Td
(59.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(2.3)Tj
13.0253 0 Td
(32.7)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(4.5)Tj
-34.9656 -1.5661 Td
(WOMEN)Tj
4.1669 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8079 0 Td
(30\))Tj
15.8529 0 Td
(44.5)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(15.6)Tj
13.0253 0 Td
(33.5)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(7.3)Tj
-34.9656 -1.5591 Td
(HIGH)Tj
2.7212 0 Td
(\()Tj
/F6 1 Tf
[( !)]TJ
/F0 1 Tf
(30\))Tj
2.5796 0 Td
(BMI)Tj
2.0055 0 Td
(male)Tj
2.374 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.815 0 Td
(7\))Tj
10.3324 0 Td
(32.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(16.2)Tj
13.0253 0 Td
(33.1)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(1.9)Tj
-34.9656 -1.5661 Td
(HIGH)Tj
2.7212 0 Td
(\()Tj
/F6 1 Tf
[( !)]TJ
/F0 1 Tf
(30\))Tj
2.5796 0 Td
(BMI)Tj
2.0055 0 Td
(female)Tj
3.2103 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8079 0 Td
(21\))Tj
9.5032 0 Td
(34.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(15.1)Tj
13.0253 0 Td
(36.1)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(6.1)Tj
-34.9656 -1.5591 Td
(HIGH)Tj
2.7212 0 Td
(\()Tj
/F6 1 Tf
[( !)]TJ
/F0 1 Tf
(30\))Tj
2.5796 0 Td
(BMI)Tj
2.0055 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8079 0 Td
(28\))Tj
12.7135 0 Td
(55.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(15.1)Tj
4.4008 0 Td
(5)Tj
8.6245 0 Td
(34.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(15.)Tj
3.848 0 Td
(4)Tj
-38.8136 -1.5662 Td
(LOW)Tj
2.5015 0 Td
(\()Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(30\))Tj
2.5796 0 Td
(BMI)Tj
2.0055 0 Td
(male)Tj
2.3811 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8079 0 Td
(23\))Tj
10.5521 0 Td
(55.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(14.7)Tj
13.0253 0 Td
(23.0)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(3.2)Tj
-34.9656 -1.559 Td
(LOW)Tj
2.5015 0 Td
(\()Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(30\))Tj
2.5796 0 Td
(BMI)Tj
2.0055 0 Td
(female)Tj
3.2103 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8079 0 Td
(9\))Tj
9.7229 0 Td
(57.5)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(16.0)Tj
13.0253 0 Td
(27.5)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(1.4)Tj
-34.9656 -1.5662 Td
(LOW)Tj
2.5015 0 Td
(\()Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(30\))Tj
2.5796 0 Td
(BMI)Tj
2.0055 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8079 0 Td
(32\))Tj
12.9332 0 Td
(35.36)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(5.7)Tj
4.4008 0 Td
(3)Tj
8.6245 0 Td
(25.3)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(3.3)Tj
-34.9656 -1.559 Td
(TOTAL)Tj
3.4441 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8149 0 Td
(60\))Tj
16.5687 0 Td
(44.5)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(15.3)Tj
13.0253 0 Td
(29.2)Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
(7.7)Tj
-34.9656 -2.3457 Td
(Urine)Tj
2.5937 0 Td
(samples)Tj
3.926 0 Td
(were)Tj
2.3811 0 Td
(collected)Tj
4.1457 0 Td
(from)Tj
2.2252 0 Td
(60)Tj
1.3323 0 Td
(individual)Tj
4.1174 0 Td
(s)Tj
0.7299 0 Td
(divided)Tj
3.3662 0 Td
(in)Tj
0.9992 0 Td
(four)Tj
1.9417 0 Td
(groups:)Tj
3.5434 0 Td
(young)Tj
2.9339 0 Td
(men,)Tj
2.4378 0 Td
(elderly)Tj
3.1464 0 Td
(men,)Tj
2.4449 0 Td
(young)Tj
-42.2648 -1.5024 Td
(women,)Tj
3.7063 0 Td
(and)Tj
1.885 0 Td
(elderly)Tj
3.1465 0 Td
(women)Tj
3.4229 0 Td
(\(n)Tj
1.1126 0 Td
(=)Tj
0.8149 0 Td
(15)Tj
1.3323 0 Td
(per)Tj
1.6583 0 Td
(group\),)Tj
3.3804 0 Td
(and)Tj
1.8779 0 Td
(also)Tj
2.0481 0 Td
(stratied)Tj
3.9898 0 Td
(by)Tj
1.2827 0 Td
(BMI,)Tj
2.2819 0 Td
(high)Tj
2.0976 0 Td
(\()Tj
/F6 1 Tf
[( !)]TJ
/F0 1 Tf
1.1481 0 Td
(30\))Tj
1.6582 0 Td
(and)Tj
1.8851 0 Td
(low)Tj
1.715 0 Td
(\()Tj
/F6 1 Tf
[( )]TJ
/F0 1 Tf
1.1409 0 Td
(30\).)Tj
1.9418 0 Td
(The)Tj
-43.5263 -1.4953 Td
(data)Tj
2.1614 0 Td
(on)Tj
1.3323 0 Td
(age)Tj
1.8779 0 Td
(and)Tj
1.878 0 Td
(BMI)Tj
2.0055 0 Td
(are)Tj
1.6654 0 Td
(presented)Tj
4.6418 0 Td
(as)Tj
1.2827 0 Td
(the)Tj
1.6086 0 Td
(median)Tj
/F6 1 Tf
3.4796 0 Td
[( )]TJ
/F0 1 Tf
(STD.)Tj
3.0473 0 Td
(BMI,)Tj
2.2819 0 Td
(body)Tj
2.3811 0 Td
(mass)Tj
2.6079 0 Td
(index.)Tj
/F8 1 Tf
-0.005 Tc
-32.2514 -2.2536 Td
(doi:)Tj
-0.025 Tc
(10.13)Tj
3.5575 0 Td
(71/journal.pone)Tj
5.8039 0 Td
(.0168188.t001)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 442.0346 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Urinary)Tj
3.3803 0 Td
(Lipidome)Tj
4.2024 0 Td
(in)Tj
0.9779 0 Td
(Healthy)Tj
3.5505 0 Td
(Popula)Tj
3.0472 0 Td
(tion)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.4748 0 Td
(DOI:10.137)Tj
5.0387 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.0168)Tj
3.5434 0 Td
(188)Tj
2.6362 0 Td
(December)Tj
4.7622 0 Td
(14,)Tj
1.5874 0 Td
(2016)Tj
36.7515 0 Td
(3)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
55 0 obj
<>stream
endstream
endobj
56 0 obj
<>stream
endstream
endobj
57 0 obj
<>stream
endstream
endobj
58 0 obj
<>stream
endstream
endobj
59 0 obj
<>stream
BT
66.402 0 Td
(13)Tj
ET
Q
endstream
endobj
60 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 11 0 R/Annots 61 0 R/Contents 62 0 R/TrimBox[0 0 612 792]>>
endobj
61 0 obj
[63 0 R 64 0 R 65 0 R 66 0 R 67 0 R 68 0 R 69 0 R 70 0 R 71 0 R 72 0 R 73 0 R 74 0 R 75 0 R 76 0 R]
endobj
63 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s003)>>
endobj
64 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s003)>>
endobj
65 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s004)>>
endobj
66 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s004)>>
endobj
67 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref018)>>
endobj
68 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.ref016)>>
endobj
69 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s005)>>
endobj
70 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s006)>>
endobj
71 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s007)>>
endobj
72 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s008)>>
endobj
73 0 obj
<>/Border[0 0 0]/A 77 0 R>>
endobj
77 0 obj
<>
endobj
74 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s006)>>
endobj
75 0 obj
<>/Border[0 0 0]/Dest(Rpone.0168188.s008)>>
endobj
76 0 obj
<>/Border[0 0 0]/A 78 0 R>>
endobj
78 0 obj
<>
endobj
62 0 obj
[79 0 R 80 0 R 81 0 R 82 0 R 83 0 R 84 0 R 85 0 R 86 0 R 87 0 R 88 0 R 89 0 R 90 0 R 91 0 R]
endobj
79 0 obj
<>stream
q
0.83 0.64 0.02 0 k
346.0535 667.3323 m
379.9559 667.3323 l
h
f*
517.5496 641.3102 m
551.452 641.3102 l
h
f*
333.5811 615.3449 m
367.5402 615.3449 l
h
f*
263.5087 504.737 m
298.2047 504.737 l
h
f*
438.2362 446.1165 m
447.7606 446.1165 l
h
f*
465.8457 407.1118 m
475.3134 407.1118 l
h
f*
277.5118 368.1071 m
287.0362 368.1071 l
h
f*
306.4819 368.1071 m
316.063 368.1071 l
h
f*
203.3575 277.115 m
212.8252 277.115 l
h
f*
232.0441 277.115 m
241.5685 277.115 l
h
f*
475.8803 277.115 m
560.7496 277.115 l
h
f*
233.8016 140.5417 m
243.326 140.5417 l
h
f*
262.8283 140.5417 m
272.3528 140.5417 l
h
f*
471.2315 94.9039 m
550.715 94.9039 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(fraction)Tj
3.3561 0 Td
(were)Tj
2.1204 0 Td
(measured)Tj
4.1102 0 Td
(by)Tj
1.1848 0 Td
(UPLC/MS/MS)Tj
6.1115 0 Td
(using)Tj
2.3867 0 Td
(a)Tj
0.6577 0 Td
(5500QTRAP)Tj
5.3404 0 Td
(quadrupole-linear)Tj
7.4551 0 Td
(ion)Tj
1.542 0 Td
(trap)Tj
-34.2649 -1.3039 Td
(hybrid)Tj
2.8743 0 Td
(mass)Tj
2.194 0 Td
(spectrometer)Tj
5.4595 0 Td
(\(AB)Tj
1.8255 0 Td
(Sciex,)Tj
2.5001 0 Td
(Framingham,)Tj
5.6579 0 Td
(MA\))Tj
2.1373 0 Td
(interfaced)Tj
4.2293 0 Td
(with)Tj
1.9956 0 Td
(an)Tj
1.2019 0 Td
(ACQUITY)Tj
4.6317 0 Td
(UPLC)Tj
-34.7071 -1.2982 Td
(System)Tj
3.05 0 Td
(\(Waters\).)Tj
3.9798 0 Td
(Transitions)Tj
4.7962 0 Td
(were)Tj
2.1203 0 Td
(determined)Tj
4.8529 0 Td
(for)Tj
1.389 0 Td
(all)Tj
1.1452 0 Td
(individual)Tj
4.2859 0 Td
(PUFAs)Tj
3.0898 0 Td
(and)Tj
1.7291 0 Td
(oxylipins)Tj
3.8551 0 Td
(using)Tj
-34.2933 -1.2983 Td
(MRM)Tj
2.6248 0 Td
(mode)Tj
2.5002 0 Td
(\(further)Tj
3.3675 0 Td
(information)Tj
5.074 0 Td
(in)Tj
0.83 0.64 0.02 0 k
1.0375 0 Td
(S2)Tj
1.1735 0 Td
(Table)Tj
0 g
(\).)Tj
3.0047 0 Td
(Because)Tj
3.3902 0 Td
(tetranor-prostaglandin)Tj
9.3543 0 Td
(D)Tj
0.9524 0 Td
(metabolite)Tj
-32.4791 -1.2983 Td
(\(tPGDM\))Tj
4.0988 0 Td
(and)Tj
1.7348 0 Td
(tetranor-prostaglandin)Tj
9.3486 0 Td
(E)Tj
0.788 0 Td
(metabolite)Tj
4.4447 0 Td
(\(tPGEM\))Tj
3.9345 0 Td
(are)Tj
1.4457 0 Td
(constitutional)Tj
5.7543 0 Td
(isomers)Tj
3.3335 0 Td
(with)Tj
-34.8829 -1.3039 Td
(indistinguishable)Tj
7.0638 0 Td
(precursor)Tj
4.0932 0 Td
(and)Tj
1.7292 0 Td
(product)Tj
3.3959 0 Td
(ion)Tj
1.542 0 Td
(mass)Tj
2.194 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.474 0 Td
(same)Tj
2.2564 0 Td
(retention)Tj
3.9061 0 Td
(time)Tj
2.0296 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(S2)Tj
1.508 0 Td
(Table)Tj
0 g
(\),)Tj
3.0104 0 Td
(it)Tj
-35.9317 -1.2982 Td
(was)Tj
1.6951 0 Td
(not)Tj
1.576 0 Td
(possible)Tj
3.4129 0 Td
(to)Tj
1.0205 0 Td
(distinguish)Tj
4.6431 0 Td
(them)Tj
2.2847 0 Td
(using)Tj
2.3924 0 Td
(our)Tj
1.6271 0 Td
(LC/MS/MS)Tj
4.8132 0 Td
(method.)Tj
3.549 0 Td
(The)Tj
1.7858 0 Td
(recovery)Tj
3.651 0 Td
(of)Tj
1.0148 0 Td
(analytes)Tj
-33.4656 -1.2983 Td
(after)Tj
2.0352 0 Td
(extraction)Tj
4.2576 0 Td
(is)Tj
0.8447 0 Td
(summarized)Tj
5.1874 0 Td
(in)Tj
0.83 0.64 0.02 0 k
1.0318 0 Td
(S3)Tj
1.1736 0 Td
(Table)Tj
0 g
(.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 587.7921 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Calibration)Tj
4.9275 0 Td
(curve)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 570.7842 cm
BT
/F5 1 Tf
1 TL
0.007 Tc
0 0 Td
(The)Tj
1.8141 0 Td
(external)Tj
3.5206 0 Td
(calibration)Tj
4.6431 0 Td
(curves)Tj
2.846 0 Td
(were)Tj
2.1657 0 Td
(constructed)Tj
5.0513 0 Td
(by)Tj
1.2075 0 Td
(spiking)Tj
3.2202 0 Td
(the)Tj
1.5023 0 Td
(sample)Tj
3.0841 0 Td
(buffer)Tj
2.6929 0 Td
(\(n)Tj
1.1338 0 Td
(=)Tj
0.8107 0 Td
(3\))Tj
1.0545 0 Td
(with)Tj
-34.7468 -1.2982 Td
(ten)Tj
1.525 0 Td
(different)Tj
3.753 0 Td
(concentrations)Tj
6.3326 0 Td
(\(0.01)Tj
2.2677 0 Td
(or)Tj
1.1225 0 Td
(0.04400)Tj
3.9004 0 Td
(or)Tj
1.1226 0 Td
(1000)Tj
2.1656 0 Td
(nM\))Tj
2.0353 0 Td
(of)Tj
1.0375 0 Td
(analytes.)Tj
3.7417 0 Td
(Analyte)Tj
3.3902 0 Td
(concentra-)Tj
-32.3941 -1.2983 Td
(tions)Tj
2.2563 0 Td
(are)Tj
1.4797 0 Td
(calculated)Tj
4.3143 0 Td
(as)Tj
1.0375 0 Td
(X)Tj
0.8844 0 Td
(\(nM\))Tj
2.3867 0 Td
(to)Tj
1.0488 0 Td
(substitute)Tj
4.1953 0 Td
([\(IS)Tj
1.7631 0 Td
(normalized)Tj
4.8869 0 Td
(analyte)Tj
3.1351 0 Td
(area\)/\(extraction)Tj
7.1149 0 Td
(recov-)Tj
-34.503 -1.3039 Td
(ery\)])Tj
2.2053 0 Td
(for)Tj
1.4173 0 Td
(Y)Tj
0.8617 0 Td
(in)Tj
1.0602 0 Td
(polynomial)Tj
4.8755 0 Td
(expressions.)Tj
5.1817 0 Td
(The)Tj
1.8199 0 Td
(calibration)Tj
4.6431 0 Td
(data,)Tj
2.194 0 Td
(including)Tj
4.1386 0 Td
(the)Tj
1.508 0 Td
(polynomial)Tj
-29.9053 -1.2983 Td
(expression)Tj
4.575 0 Td
(of)Tj
1.0375 0 Td
(the)Tj
1.5024 0 Td
(approximate)Tj
5.3914 0 Td
(curve,)Tj
2.7156 0 Td
(calibration)Tj
4.6431 0 Td
(range,)Tj
2.7553 0 Td
(and)Tj
1.7631 0 Td
(correlation)Tj
4.7395 0 Td
(coefficient)Tj
4.4957 0 Td
(are)Tj
1.4854 0 Td
(all)Tj
-35.104 -1.2982 Td
(summarized)Tj
5.295 0 Td
(in)Tj
0.83 0.64 0.02 0 k
1.0545 0 Td
(S3)Tj
1.1962 0 Td
(Table)Tj
0 g
(.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 477.1842 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Data)Tj
2.3102 0 Td
(processing)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 460.1763 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(LC-TOFMS)Tj
5.0229 0 Td
(data)Tj
1.9162 0 Td
(were)Tj
2.1203 0 Td
(processed)Tj
4.1386 0 Td
(using)Tj
2.3867 0 Td
(the)Tj
1.4684 0 Td
(2DICAL)Tj
3.6623 0 Td
(software)Tj
3.583 0 Td
(package)Tj
3.4185 0 Td
(\(Mitsui)Tj
3.1692 0 Td
(Knowledge)Tj
-30.8861 -1.2982 Td
(Industry)Tj
3.651 0 Td
(Co.)Tj
1.6157 0 Td
(Ltd.,)Tj
2.0296 0 Td
(Tokyo,)Tj
3.0217 0 Td
(Japan\))Tj
2.8233 0 Td
(as)Tj
1.0148 0 Td
(described)Tj
4.0535 0 Td
(by)Tj
1.1849 0 Td
(Ono)Tj
2.0182 0 Td
(et)Tj
0.9468 0 Td
(al.)Tj
1.1225 0 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(].)Tj
2.075 0 Td
(Extracted)Tj
4.0308 0 Td
(ion)Tj
1.5477 0 Td
(peaks)Tj
2.4491 0 Td
(were)Tj
2.1203 0 Td
(sub-)Tj
-35.7049 -1.2983 Td
(jected)Tj
2.5738 0 Td
(to)Tj
1.0261 0 Td
(identification)Tj
5.5842 0 Td
(of)Tj
1.0148 0 Td
(lipid)Tj
2.0466 0 Td
(molecules)Tj
4.1953 0 Td
(by)Tj
1.1849 0 Td
(comparing)Tj
4.5694 0 Td
(ion)Tj
1.542 0 Td
(features,)Tj
3.5773 0 Td
(including)Tj
4.0365 0 Td
(RT,)Tj
1.6838 0 Td
(m/z,)Tj
2.0069 0 Td
(pre-)Tj
-35.0416 -1.2982 Td
(ferred)Tj
2.6248 0 Td
(adducts,)Tj
3.5433 0 Td
(and)Tj
1.7291 0 Td
(in-source)Tj
4.0025 0 Td
(fragments,)Tj
4.4561 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(experimental)Tj
5.4595 0 Td
(samples)Tj
3.3788 0 Td
(with)Tj
1.9956 0 Td
(those)Tj
2.3414 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
-33.0687 -1.304 Td
(reference)Tj
3.9174 0 Td
(library)Tj
2.8686 0 Td
(of)Tj
1.0148 0 Td
(lipid)Tj
2.0466 0 Td
(molecule)Tj
3.8381 0 Td
(entries,)Tj
3.1351 0 Td
(as)Tj
1.0148 0 Td
(described)Tj
4.0535 0 Td
(previously)Tj
4.354 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(].)Tj
2.075 0 Td
(The)Tj
1.7801 0 Td
(peak)Tj
2.092 0 Td
(height)Tj
2.7326 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
-35.9374 -1.2982 Td
(detected)Tj
3.5659 0 Td
(lipid)Tj
2.0466 0 Td
(molecules)Tj
4.1953 0 Td
(was)Tj
1.7007 0 Td
(corrected)Tj
3.9912 0 Td
(to)Tj
1.0205 0 Td
(that)Tj
1.7801 0 Td
(of)Tj
1.0148 0 Td
(deuterated)Tj
4.4787 0 Td
(PC)Tj
1.44 0 Td
(as)Tj
1.0148 0 Td
(an)Tj
1.2019 0 Td
(IS)Tj
1.0375 0 Td
(in)Tj
1.0374 0 Td
(each)Tj
2.024 0 Td
(sample.)Tj
3.2484 0 Td
(The)Tj
-34.7978 -1.2983 Td
(corrected)Tj
3.9911 0 Td
(value)Tj
2.3017 0 Td
(for)Tj
1.389 0 Td
(each)Tj
2.0296 0 Td
(lipid)Tj
2.0409 0 Td
(was)Tj
1.7008 0 Td
(then)Tj
2.0182 0 Td
(normalized)Tj
4.7792 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(corresponding)Tj
6.0604 0 Td
(creatinine)Tj
4.2236 0 Td
(levels)Tj
2.3755 0 Td
(in)Tj
-35.4045 -1.3039 Td
(each)Tj
2.0295 0 Td
(urine)Tj
2.3585 0 Td
(sample)Tj
3.0217 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(S4)Tj
0 g
1.508 0 Td
(and)Tj
0.83 0.64 0.02 0 k
1.7291 0 Td
(S5)Tj
0 g
1.1735 0 Td
(Tables\).)Tj
-0.0099 Tc
-10.6241 -1.2983 Td
(UPLC/MS/MS)Tj
6.0547 0 Td
(data)Tj
1.8936 0 Td
(on)Tj
1.2642 0 Td
(PUFAs)Tj
3.0557 0 Td
(and)Tj
1.7121 0 Td
(oxylipins)Tj
3.8041 0 Td
(were)Tj
2.0976 0 Td
(processed)Tj
4.0819 0 Td
(using)Tj
2.3584 0 Td
(MultiQuant)Tj
/F10 1 Tf
[()]TJ
/F5 1 Tf
5.3971 0 Td
(Software)Tj
-32.9156 -1.2982 Td
(\(Version)Tj
3.685 0 Td
(2.1;)Tj
1.5987 0 Td
(AB)Tj
1.474 0 Td
(Sciex\).)Tj
2.7949 0 Td
(The)Tj
1.7689 0 Td
(integrated)Tj
4.2179 0 Td
(peak)Tj
2.0636 0 Td
(area)Tj
1.8595 0 Td
(of)Tj
1.0035 0 Td
(the)Tj
1.4513 0 Td
(detected)Tj
3.5206 0 Td
(lipid)Tj
2.0183 0 Td
(molecules)Tj
4.1442 0 Td
(was)Tj
1.6781 0 Td
(corrected)Tj
-33.2785 -1.304 Td
(to)Tj
1.0147 0 Td
(that)Tj
1.7518 0 Td
(of)Tj
1.0035 0 Td
(deuterated)Tj
4.4164 0 Td
(LTB)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 298.9984 328.5354 cm
BT
/F5 1 Tf
1 TL
-0.0132 Tc
0 0 Td
(4)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 302.5133 330.1795 cm
BT
/F5 1 Tf
1 TL
-0.0099 Tc
0 0 Td
(-d4)Tj
1.5533 0 Td
(as)Tj
1.0035 0 Td
(an)Tj
1.1905 0 Td
(IS)Tj
1.0262 0 Td
(in)Tj
1.0204 0 Td
(each)Tj
2.0069 0 Td
(sample.)Tj
3.2032 0 Td
(The)Tj
1.7688 0 Td
(concentration)Tj
5.7429 0 Td
(in)Tj
1.0205 0 Td
(the)Tj
1.4513 0 Td
(correction-)Tj
-31.2375 -1.2982 Td
(applied)Tj
3.1125 0 Td
(samples)Tj
3.3391 0 Td
(was)Tj
1.6838 0 Td
(determined)Tj
4.7904 0 Td
(by)Tj
1.1679 0 Td
(using)Tj
2.3641 0 Td
(the)Tj
1.4513 0 Td
(calibration)Tj
4.456 0 Td
(curve)Tj
2.3925 0 Td
(and)Tj
1.7121 0 Td
(by)Tj
1.1679 0 Td
(adjusting)Tj
3.8551 0 Td
(the)Tj
1.4513 0 Td
(extraction)Tj
-32.944 -1.2983 Td
(efficiency)Tj
3.9685 0 Td
(as)Tj
1.0035 0 Td
(described)Tj
3.9968 0 Td
(above.)Tj
2.7381 0 Td
(The)Tj
1.7632 0 Td
(concentration)Tj
5.7429 0 Td
(of)Tj
1.0035 0 Td
(each)Tj
2.0069 0 Td
(lipid)Tj
2.0126 0 Td
(was)Tj
1.6838 0 Td
(then)Tj
1.9956 0 Td
(normalized)Tj
4.7168 0 Td
(to)Tj
1.0148 0 Td
(the)Tj
1.4513 0 Td
(corre-)Tj
-35.0983 -1.3039 Td
(sponding)Tj
3.9062 0 Td
(creatinine)Tj
4.1612 0 Td
(levels)Tj
2.3356 0 Td
(in)Tj
1.0148 0 Td
(each)Tj
2.0013 0 Td
(urine)Tj
2.3187 0 Td
(sample)Tj
2.982 0 Td
(and)Tj
1.7065 0 Td
(expressed)Tj
4.0308 0 Td
(as)Tj
/F10 1 Tf
0.9978 0 Td
[()]TJ
/F5 1 Tf
(mol)Tj
2.2677 0 Td
(analyte)Tj
3.016 0 Td
(/mmol)Tj
2.9027 0 Td
(creatinine)Tj
-33.6413 -1.2983 Td
(\()Tj
0.83 0.64 0.02 0 k
(S6)Tj
0 g
1.4911 0 Td
(and)Tj
0.83 0.64 0.02 0 k
1.7121 0 Td
(S7)Tj
0 g
1.1622 0 Td
(Tables\))Tj
3.101 0 Td
(as)Tj
1.0035 0 Td
(described)Tj
3.9967 0 Td
(in)Tj
1.0262 0 Td
(the)Tj
1.4513 0 Td
(Human)Tj
3.2882 0 Td
(Metabolome)Tj
5.2213 0 Td
(Database)Tj
3.7984 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(http://www.hmdb.ca/)Tj
0 g
(\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 249.6188 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(ELISA)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 232.611 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(For)Tj
1.627 0 Td
(urine)Tj
2.3584 0 Td
(volume)Tj
3.1975 0 Td
(normalization,)Tj
6.1058 0 Td
(the)Tj
1.4683 0 Td
(creatinine)Tj
4.2293 0 Td
(level)Tj
2.0126 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(urine)Tj
2.3584 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
2.0296 0 Td
(subject)Tj
3.016 0 Td
(was)Tj
1.7008 0 Td
(evaluated)Tj
-33.6243 -1.2982 Td
(by)Tj
1.1792 0 Td
(enzyme-linked)Tj
6.1624 0 Td
(immunosorbent)Tj
6.7294 0 Td
(assay)Tj
2.2621 0 Td
(\(TransGenic)Tj
5.2667 0 Td
(Inc.,)Tj
1.9843 0 Td
(Kobe,)Tj
2.5511 0 Td
(Japan\).)Tj
3.0501 0 Td
(Urine)Tj
2.5682 0 Td
(\(10)Tj
/F10 1 Tf
1.5136 0 Td
[()]TJ
/F5 1 Tf
(l\))Tj
1.3096 0 Td
(was)Tj
-34.5767 -1.3039 Td
(diluted)Tj
3.0387 0 Td
(with)Tj
1.9955 0 Td
(190)Tj
/F10 1 Tf
1.6498 0 Td
[()]TJ
/F5 1 Tf
(l)Tj
0.9694 0 Td
(reaction)Tj
3.4923 0 Td
(buffer.)Tj
2.8516 0 Td
(The)Tj
1.7859 0 Td
(diluted)Tj
3.0387 0 Td
(sample)Tj
3.0217 0 Td
(was)Tj
1.6951 0 Td
(centrifuged)Tj
4.7849 0 Td
(at)Tj
0.9524 0 Td
(1,500)Tj
2.3471 0 Td
(rpm,)Tj
2.1543 0 Td
(and)Tj
1.7291 0 Td
(70)Tj
/F10 1 Tf
[()]TJ
/F5 1 Tf
(l)Tj
-35.5065 -1.2983 Td
(of)Tj
1.0147 0 Td
(the)Tj
1.4684 0 Td
(supernatant)Tj
4.9833 0 Td
(was)Tj
1.7007 0 Td
(transferred)Tj
4.6375 0 Td
(to)Tj
1.0261 0 Td
(a)Tj
0.652 0 Td
(96)Tj
1.1735 0 Td
(well)Tj
1.8199 0 Td
(plate.)Tj
2.364 0 Td
(After)Tj
2.2847 0 Td
(addition)Tj
3.5944 0 Td
(of)Tj
1.0147 0 Td
(70)Tj
/F10 1 Tf
1.1736 0 Td
[()]TJ
/F5 1 Tf
(l)Tj
0.9751 0 Td
(of)Tj
1.0148 0 Td
(reaction)Tj
3.4923 0 Td
(solu-)Tj
-34.3897 -1.2983 Td
(tion,)Tj
2.0636 0 Td
(the)Tj
1.4683 0 Td
(plate)Tj
2.143 0 Td
(was)Tj
1.7008 0 Td
(incubated)Tj
4.1725 0 Td
(at)Tj
0.9525 0 Td
(RT)Tj
1.457 0 Td
(for)Tj
1.3833 0 Td
(1)Tj
0.6973 0 Td
(h.)Tj
0.9694 0 Td
(After)Tj
2.2904 0 Td
(washing)Tj
3.5036 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(plate,)Tj
2.3641 0 Td
(chromogenic)Tj
5.4992 0 Td
(substrate)Tj
-33.1538 -1.3039 Td
(solution)Tj
3.4809 0 Td
(\(100)Tj
/F10 1 Tf
1.9899 0 Td
[()]TJ
/F5 1 Tf
(l\))Tj
1.3096 0 Td
(was)Tj
1.7008 0 Td
(added)Tj
2.6588 0 Td
(to)Tj
1.0262 0 Td
(each)Tj
2.0296 0 Td
(well)Tj
1.8198 0 Td
(and)Tj
1.7291 0 Td
(incubated)Tj
4.1783 0 Td
(for)Tj
1.3889 0 Td
(10)Tj
1.1679 0 Td
(min.)Tj
2.0806 0 Td
(The)Tj
1.7858 0 Td
(absorption)Tj
4.5354 0 Td
(at)Tj
0.9525 0 Td
(490)Tj
1.6441 0 Td
(nm)Tj
-35.4782 -1.2982 Td
(was)Tj
1.6951 0 Td
(analyzed)Tj
3.702 0 Td
(using)Tj
2.3924 0 Td
(a)Tj
0.652 0 Td
(microplate)Tj
4.5297 0 Td
(reader.)Tj
2.999 0 Td
(Each)Tj
2.1714 0 Td
(plate)Tj
2.1373 0 Td
(comprised)Tj
4.439 0 Td
(a)Tj
0.6576 0 Td
(calibration)Tj
4.5241 0 Td
(set)Tj
1.2983 0 Td
(and)Tj
1.7348 0 Td
(solvent)Tj
-32.9327 -1.2983 Td
(blanks.)Tj
3.0387 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(S5)Tj
0 g
1.508 0 Td
(and)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(S7)Tj
0 g
1.1679 0 Td
(Tables\))Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 112.9889 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Statistical)Tj
4.4504 0 Td
(analysis)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 95.9811 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Statistical)Tj
4.0195 0 Td
(analyses)Tj
3.4865 0 Td
(were)Tj
2.1203 0 Td
(performed)Tj
4.4731 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.4683 0 Td
(R)Tj
0.8447 0 Td
(statistical)Tj
3.9062 0 Td
(environment)Tj
5.4311 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(http://r-project.org/)Tj
0 g
8.2885 0 Td
(\).)Tj
-35.07 -1.2982 Td
(Metabolite)Tj
4.5183 0 Td
(levels)Tj
2.3755 0 Td
(among)Tj
2.9933 0 Td
(the)Tj
1.4684 0 Td
(four)Tj
1.9162 0 Td
(groups)Tj
2.965 0 Td
(were)Tj
2.126 0 Td
(compared)Tj
4.2406 0 Td
(by)Tj
1.1848 0 Td
(the)Tj
1.4684 0 Td
(Mann-Whitney)Tj
6.4799 0 Td
(test,)Tj
1.8255 0 Td
(and)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 442.0346 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Urinary)Tj
3.3803 0 Td
(Lipidome)Tj
4.2024 0 Td
(in)Tj
0.9779 0 Td
(Healthy)Tj
3.5505 0 Td
(Popula)Tj
3.0472 0 Td
(tion)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.4748 0 Td
(DOI:10.137)Tj
5.0387 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.0168)Tj
3.5434 0 Td
(188)Tj
2.6362 0 Td
(December)Tj
4.7622 0 Td
(14,)Tj
1.5874 0 Td
(2016)Tj
36.7515 0 Td
(4)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
80 0 obj
<>stream
endstream
endobj
81 0 obj
<>stream
endstream
endobj
82 0 obj
<>stream
endstream
endobj
83 0 obj
<>stream
endstream
endobj
84 0 obj
<>stream
endstream
endobj
85 0 obj
<>stream
endstream
endobj
86 0 obj
<>stream
endstream
endobj
87 0 obj
<>stream
endstream
endobj
88 0 obj
<>stream
endstream
endobj
89 0 obj
<>stream
endstream
endobj
90 0 obj
<>stream
endstream
endobj
91 0 obj
<>stream
BT
66.402 0 Td
(13)Tj
ET
Q
endstream
endobj
92 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 11 0 R/Annots 98 0 R/Contents 99 0 R/TrimBox[0 0 612 792]>>
endobj
94 0 obj
<>
endobj
100 0 obj
<>
endobj
103 0 obj
<>stream
MinionPro-It 9 `ab]+
W W Y ' - 3 > H N T Z ` j q t { +2=CIU[cnz~
"(.9CIOU[elov|&-8>DPV^iuy &+/49@DGLPU[]_ahjmru|
&-4;BIPW^elsz
")07>ELSZahov} %,3:AHOV]dkry#/;JTey ! ( / 6 = D K R Y ` g n u |
$
+
2
9
@
G
N
U
\
c
j
q
x
'.5<CJQX_fmt{ #*18?FMT[bkry
"
)
0
7
>
E
J
O
W
d
q
v
$+29@GNU\cjqx '.5<CJQX_fmt{ #*18?FMT[bipw~
&-4;BIPW^elsz !(/6=DOVcpuz-B\v$<Tdy*5@Tanz!2>O_fmt{ #*18?FMT[bipw~(4@LZk{ "3EU_px!-?IUas $6BN\m
-:EQ]n}
%.7@IR[dmv!*3<ENW`ir{&/8AJS\enw ! * 3 < E N W ` i r { !!
!""commaaccentAbreveAmacronAogonekAEacuteCacuteCcaronCcircumflexCdotaccentDcaronDcroatEbreveEcaronEdotaccentEmacronEngEogonekGbreveGcircumflexGcommaaccentGdotaccentHbarHcircumflexIbreveIJImacronIogonekItildeJcircumflexKcommaaccentLacuteLcaronLcommaaccentLdotNacuteNcaronNcommaaccentObreveOhungarumlautOmacronOslashacuteRacuteRcaronRcommaaccentSacuteScedillaScircumflexScommaaccentTbarTcaronTcommaaccentUbreveUhungarumlautUmacronUogonekUringUtildeWacuteWcircumflexWdieresisWgraveYcircumflexYgraveZacuteZdotaccentIdotaccentabreveamacronaogonekaeacutecacuteccaronccircumflexcdotaccentdcarondcroatebreveecaronedotaccentemacronengeogonekgbrevegcircumflexgcommaaccentgdotaccenthbarhcircumflexibreveijimacroniogonekitildejcircumflexkcommaaccentlacutelcaronlcommaaccentldotnacutencaronncommaaccentobreveohungarumlautomacronoslashacuteracutercaronrcommaaccentsacutescedillascircumflexscommaaccenttbartcarontcommaaccentubreveuhungarumlautumacronuogonekuringutildewacutewcircumflexwdieresiswgraveycircumflexygravezacutezdotaccentkgreenlandiclongsnapostrophetonosdieresistonosAlphaBetaGammaDeltaEpsilonZetaEtaThetaIotaKappaLambdaMuNuXiOmicronPiRhoSigmaTauUpsilonPhiChiPsiOmegaAlphatonosEpsilontonosEtatonosIotatonosIotadieresisOmicrontonosUpsilontonosUpsilondieresisOmegatonosuni1FBBuni1F09uni1F0Duni1F0Buni1F0Funi1FB8uni1FBAuni1FBCuni1F89uni1F8Duni1F8Buni1F8Funi1F88uni1F8Cuni1F8Auni1F8Euni1F08uni1F0Cuni1F0Auni1F0Euni1FB9uni1FC9uni1F19uni1F1Duni1F1Buni1FC8uni1F18uni1F1Cuni1F1Auni1FCBuni1F29uni1F2Duni1F2Buni1F2Funi1FCAuni1FCCuni1F99uni1F9Duni1F9Buni1F9Funi1F98uni1F9Cuni1F9Auni1F9Euni1F28uni1F2Cuni1F2Auni1F2Euni1FDBuni1F39uni1F3Duni1F3Buni1F3Funi1FD8uni1FDAuni1F38uni1F3Cuni1F3Auni1F3Euni1FD9uni1FFBuni1F69uni1F6Duni1F6Buni1F6Funi1FFAuni1FFCuni1FA9uni1FADuni1FABuni1FAFuni1FA8uni1FACuni1FAAuni1FAEuni1F68uni1F6Cuni1F6Auni1F6Euni1FF9uni1F49uni1F4Duni1F4Buni1FF8uni1F48uni1F4Cuni1F4Auni1FECuni1FEBuni1F59uni1F5Duni1F5Buni1F5Funi1FE8uni1FEAuni1FE9alphabetagammadeltaepsilonzetaetathetaiotakappalambdanuxiomicronpirhosigmatauupsilonphichipsiomegaalphatonosepsilontonosetatonosiotatonosiotadieresisomicrontonosupsilontonosupsilondieresisomegatonosiotadieresistonosupsilondieresistonosuni03C2uni0374uni0375uni037Euni03D0uni03DAuni03DEuni03E0uni03F0uni1FBDuni1FBEuni1FC1uni1FCDuni1FCEuni1FCFuni1FDDuni1FDEuni1FDFuni1FEDuni1FEEuni1FFEuni1F00uni1F01uni1F02uni1F03uni1F04uni1F05uni1F06uni1F07uni1F10uni1F11uni1F12uni1F13uni1F14uni1F15uni1F20uni1F21uni1F22uni1F23uni1F24uni1F25uni1F26uni1F27uni1F30uni1F31uni1F32uni1F33uni1F34uni1F35uni1F36uni1F37uni1F40uni1F41uni1F42uni1F43uni1F44uni1F45uni1F50uni1F51uni1F52uni1F53uni1F54uni1F55uni1F56uni1F57uni1F60uni1F61uni1F62uni1F63uni1F64uni1F65uni1F66uni1F67uni1F70uni1F71uni1F72uni1F73uni1F74uni1F75uni1F76uni1F77uni1F78uni1F79uni1F7Auni1F7Buni1F7Cuni1F7Duni1F80uni1F81uni1F82uni1F83uni1F84uni1F85uni1F86uni1F87uni1F90uni1F91uni1F92uni1F93uni1F94uni1F95uni1F96uni1F97uni1FA0uni1FA1uni1FA2uni1FA3uni1FA4uni1FA5uni1FA6uni1FA7uni1FB0uni1FB1uni1FB2uni1FB3uni1FB6uni1FB7uni1FC2uni1FC3uni1FC4uni1FC6uni1FC7uni1FD0uni1FD1uni1FD2uni1FD3uni1FD6uni1FD7uni1FE0uni1FE1uni1FE2uni1FE3uni1FE4uni1FE5uni1FE6uni1FF2uni1FF3uni1FF4uni1FF6uni1FF7uni1FB4uni1FE7uniEFD5anoteleiauni03D1uni03D5uni03D6franclirapesetaEuroafii61289estimatedpartialdiffproductsummationuni2219radicalinfinityintegralapproxequalnotequallessequalgreaterequallozengeuni02C9uniEFEDuniEFEEuniEFF1uniEFF2uniEFF3uniEFF5uniEFF7AcuteCaronDieresisDieresisAcuteDieresisGraveGraveHungarumlautMacroncyrBrevecyrFlexdblGravecyrbrevecyrflexdblgravedieresisacutedieresisgraveuniF635uniF637uniF638uniF639uniF6DCuniF63AuniF63BuniF63CuniF63DuniF63EuniF63FuniF640uniF641uniF642uniF628uniF643uniF644uniF645uniF646uniF647uniF648uniF649uniF64AuniF64BuniF64CuniF64DuniF64EuniF64FuniF650uniF651uniF652uniF653uniF654uniF655uniF656uniF657uniF658uniF659uniF65AuniF65BuniF65CuniF65DuniF65EuniF65FuniF660uniF629uniF62AuniF62BuniF62CuniF62DuniF661uniF662uniF663uniF664uniF665uniF666uniF667uniF668uniF669uniF66AuniF66BuniF66CuniF62EuniF62FuniF630uniF631uniF632uniF66DuniF66EuniF66FuniF670uniF671uniF672uniF673uniF674uniF675uniF676uniF677uniF678uniF679uniF67AuniF67BuniF67CuniF67DuniF67EuniF67FuniF680uniF681uniF682uniF683uniF684uniF685uniF686uniF687uniF688uniF689uniF68AuniF68BuniF68CuniF68DuniF68EuniF68FuniF690uniF691uniF692uniF693uniF694uniF695uniF696uniF697uniF698uniF699uniF69AuniF69BuniF69CuniF69DuniF69EuniF69FuniF6A0uniF6A1uniF6A2uniF6A3uniF6A4uniF6A5uniF6A6uniF6A7uniF6A8uniF6A9uniF6AAuniF6ABuniF6ACuniF6ADuniF6AEuniF6AFuniF6B0uniF6B1uniF6B2uniF6B3uniF6B4uniF6B5uniF6B6uniF6B7uniF6B8uniF6B9uniF6BAuniF6BBuniF6BCuniF6BDuniF633c_hc_kc_tf_bf_f_bf_f_hf_f_kf_f_tf_hf_jf_f_jf_kf_ts_puniFB06T_hlongs_blongs_hlongs_ilongs_klongs_llongs_longsuniFB05longs_longs_ilongs_longs_la.ende.endm.endn.endr.endK.altR.altk.altcaron.altcommaaccent.altpeseta.altAlphaiotasub.altAlphaiotasubasper.altAlphaiotasubasperacute.altAlphaiotasubaspergrave.altAlphaiotasubaspertilde.altAlphaiotasublenis.altAlphaiotasublenisacute.altAlphaiotasublenisgrave.altAlphaiotasublenistilde.altEtaiotasub.altEtaiotasubasper.altEtaiotasubasperacute.altEtaiotasubaspergrave.altEtaiotasubaspertilde.altEtaiotasublenis.altEtaiotasublenisacute.altEtaiotasublenisgrave.altEtaiotasublenistilde.altOmegaiotasub.altOmegaiotasubasper.altOmegaiotasubasperacute.altOmegaiotasubaspergrave.altOmegaiotasubaspertilde.altOmegaiotasublenis.altOmegaiotasublenisacute.altOmegaiotasublenisgrave.altOmegaiotasublenistilde.altEuro.fittedcent.fittedcolonmonetary.fitteddollar.fittedflorin.fittedfranc.fittedlira.fittednumbersign.fittedsterling.fittedyen.fittedEuro.oldstylecolonmonetary.oldstyleestimated.oldstyleflorin.oldstylefranc.oldstylelira.oldstylenumbersign.oldstylesterling.oldstyleyen.oldstylefranc.taboldstylelira.taboldstyleorn.001orn.002orn.003orn.004orn.005orn.006orn.007orn.008orn.009orn.010orn.011orn.012orn.013orn.014orn.015orn.016orn.017orn.018orn.019orn.020orn.021orn.022orn.023A.swashB.swashC.swashD.swashE.swashF.swashG.swashH.swashI.swashJ.swashK.swashL.swashM.swashN.swashO.swashP.swashQ.swashR.swashS.swashT.swashU.swashV.swashW.swashX.swashY.swashZ.swashT_h.swashAacute.swashAbreve.swashAcircumflex.swashAdieresis.swashAgrave.swashAmacron.swashAogonek.swashAring.swashAtilde.swashCacute.swashCcaron.swashCcedilla.swashCcircumflex.swashCdotaccent.swashDcaron.swashDcroat.swashEacute.swashEbreve.swashEcaron.swashEcircumflex.swashEdieresis.swashEdotaccent.swashEgrave.swashEmacron.swashEng.swashEogonek.swashGbreve.swashGcircumflex.swashGcommaaccent.swashGdotaccent.swashHbar.swashHcircumflex.swashIJ.swashIacute.swashIbreve.swashIcircumflex.swashIdieresis.swashIdotaccent.swashIgrave.swashImacron.swashIogonek.swashItilde.swashJcircumflex.swashKcommaaccent.swashLacute.swashLcaron.swashLcommaaccent.swashLdot.swashNacute.swashNcaron.swashNcommaaccent.swashNtilde.swashOacute.swashObreve.swashOcircumflex.swashOdieresis.swashOgrave.swashOhungarumlaut.swashOmacron.swashOslash.swashOslashacute.swashOtilde.swashRacute.swashRcaron.swashRcommaaccent.swashSacute.swashScaron.swashScedilla.swashScircumflex.swashScommaaccent.swashTbar.swashTcaron.swashTcommaaccent.swashT_h.caronswashT_h.commaaccentswashUacute.swashUbreve.swashUcircumflex.swashUdieresis.swashUgrave.swashUhungarumlaut.swashUmacron.swashUogonek.swashUring.swashUtilde.swashWacute.swashWcircumflex.swashWdieresis.swashWgrave.swashYacute.swashYcircumflex.swashYdieresis.swashYgrave.swashZacute.swashZcaron.swashZdotaccent.swashafii10056T_h.caronT_h.commaaccentafii10048afii10074afii10075afii10082afii10192afii10062afii10194afii10193afii10102afii10071afii10041afii10076afii10085afii10103afii10051afii10039afii10037afii10079afii10061afii10059afii10047afii10067afii10049afii10148afii10033afii10094afii10080afii10022afii10145afii10063afii10027afii10070afii10054afii10109afii10036afii10065afii10055afii10068afii10086afii10064afii10096afii10050afii10146afii10077afii10097afii10069afii10107afii10101afii10043afii10078afii10024afii10073afii10089afii10092afii10147afii10087afii10081afii10029afii10083afii10026afii10019afii10032afii10046afii10072afii10021afii10030afii10098afii10038afii10017afii10040afii10042afii10105afii10052afii10057afii10025afii10034afii10053afii10832afii10058afii10018afii10044afii10110afii10020afii10095Rholenisafii10104afii10106afii10090afii10091afii10060afii10028afii10045afii10099afii10066afii10084afii10031afii10196afii10035afii10088afii10093Eth.swashafii10100uni0394afii10023afii10195uni2215uni021Aafii10108uni03BCuni00ADuni00A0uni03A9uni021Bafii10846afii61352afii10831Copyright 2000 Adobe Systems Incorporated. All Rights Reserved. U.S. Patents Des. 338,907 and 371,799.Minion is either a registered trademark or a trademark of Adobe Systems Incorporated in the United States and/or other countries.Minion Pro ItalicMinion Proq N s ^mTw6zgjTRC @ q
C
d
m
r$7
,
Q
c
!*D )d Gcw~!;FJ3%3@FP8ir3y1S_ju(/6:n . 9 C v !!/!5!`!h!!!!!"""@"g"t"|""""###W#\#h#n#v######$$ $&$,$3$:$K$U$r$$$$$$$$$% %%%5%D%]%h%o%v%%%%%%%%%%%& &&,&9&C&J&P&U&j&&&&&&&&&&&''''#'(':'L'Q'\'a'g'x'''''''''(((%(/(7(>(E(L([(j(w(~((((((((((((((())))!).)9)D)K)R)Y)`)f)k)o)t))))))))))))))**
****%*0*;*F*Q*\*g*r*}*****-
oFl\8Mgk^?ŜeQ[a`FpUI߹|{{|VsrocghpzvL##4/Jcݹ+!MWagpu
|;;.|;9hꖧovgtfstqon@Sm|}ĬD⋄DQnJs8*Tlb~wg8`F(;@§ŰY9kde:S!VtVl-ܲq
m
ބ}A98}@=
^98ԏڒ,`fhMhx{r/S#Qݟ9ʞJ+^VT\]xZ]Rmh
dn$d_JoDO(q
zm
ӇbjVy-}EVeF4)AKڑף
ڄ|>[tA_d}ʟɩ藿ΎwC{LZnXH;slz`Z?; :\o"T÷ķT`ź_j{xR{YvPwUBy
|s^`_ZNu[XUoaN{;iV`VsjaK]<_IZF"v,
b|bw`tgigxumjbisˎA{lW_ïknruxutnivry?y>x? rxǼiwi4wxηŻ6xyppvTyJyQ,|;7
^1؟svmdؓd
upՆ]oXy.]#m
vu|YWx.^h~_tWpy^Fy_pp͆SVQL
owÐˑ_Dm
{qtg`O_Rxo|TpgC]cwxz8REpzD掚˴k[uqlchkmXfjevrkpocjfq~z~|zhfsPfnwr
Wym{qhlb\2\)FWy:y1er}yo;ڕԑZhĚڰW-Änj~sypkWkuo_8lewߢۂ|ogYQZktY00=ZH<4 Eu{tzy{|wB/`J
aoAh{1~Ug^YoG=E)?XEݻd9N9&)^:<>